PA 1 dihydrochloride

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Cat.No. 5463 - PA 1 dihydrochloride | C18H16ClN9O.2HCl
Description: Photoswitchable ENaC blocker
Chemical Name: 3,5-Diamino-6-chloro-N-[imino[[4-(2-phenyldiazenyl)phenyl]amino]methyl]-2-pyrazinecarboxamide dihydrochloride
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity

Photoswitchable epithelial sodium channel (ENaC) blocker (IC50 values are 90 and 390 nM for αβγ and δβγENaCs, respectively, in the trans conformation). Switches conformation from cis to trans at 500 nm and trans to cis at 400 nm. Blocks ENaCs with greater efficacy in the cis conformation, in comparison to the trans conformation, in Xenopus oocytes and HEK293t cells. Partially blocks δβγENaCs in the trans conformation and exhibits near maximal block of δβγENaCs in the cis conformation. Amiloride derivative.

Technical Data

M. Wt 482.75
Formula C18H16ClN9O.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
PubChem ID 121513862
Smiles O=C(NC(NC2=CC=C(/N=N/C3=CC=CC=C3)C=C2)=N)C1=C(N)N=C(N)C(Cl)=N1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 48.27 100

Preparing Stock Solutions

The following data is based on the product molecular weight 482.75. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.07 mL 10.36 mL 20.71 mL
5 mM 0.41 mL 2.07 mL 4.14 mL
10 mM 0.21 mL 1.04 mL 2.07 mL
50 mM 0.04 mL 0.21 mL 0.41 mL

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References are publications that support the biological activity of the product.

Schönberger et al (2014) Controlling epithelial sodium channels with light using photoswitchable amilorides. Nat.Chem. 6 712 PMID: 25054942

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Keywords: PA 1 dihydrochloride, PA 1 dihydrochloride supplier, photoswitchable, ENaC, blockers, epithelial, sodium, channels, Na+, photoamiloride-1, PA1, ligands, Photoswitchable, Ligands, Epithelial, Sodium, Channels, 5463, Tocris Bioscience

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