ONO AE3 208

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Description: High affinity and selective EP4 antagonist
Chemical Name: 4-Cyano-2-[[2-(4-fluoro-1-naphthalenyl)-1-oxopropyl]amino]benzenebutanoic acid
Purity: ≥98% (HPLC)
Citations (2)

Biological Activity for ONO AE3 208

ONO AE3 208 is a high affinity and selective EP4 receptor antagonist (Ki values are 1.3, 30, 790 and 2400 nM for EP4, EP3, FP and TP receptors respectively). Displays no affinity for EP1, EP2, DP or IP receptors (Ki >10 μM). Inhibits PGE2-induced IL-8 production in colonic epithelial caco-2 cells and attenuates PGE2 inhibition of natural killer T cell activation. Suppresses recovery from experimentally-induced colitis and stimulates CD4+ T cell proliferation in C57BL/6 mice. Also reduces metastasis of mammary tumor cells in a murine model of breast cancer. Orally active.

Technical Data for ONO AE3 208

M. Wt 404.43
Formula C24H21FN2O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 402473-54-5
PubChem ID 10111831
Smiles O=C(C(C)C2=C(C=CC=C3)C3=C(F)C=C2)NC1=CC(C#N)=CC=C1CCCC(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ONO AE3 208

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 40.44 100

Preparing Stock Solutions for ONO AE3 208

The following data is based on the product molecular weight 404.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.47 mL 12.36 mL 24.73 mL
5 mM 0.49 mL 2.47 mL 4.95 mL
10 mM 0.25 mL 1.24 mL 2.47 mL
50 mM 0.05 mL 0.25 mL 0.49 mL

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Product Datasheets for ONO AE3 208

Certificate of Analysis / Product Datasheet
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References for ONO AE3 208

References are publications that support the biological activity of the product.

Ma et al (2006) Prostaglandin E receptor EP4 antagonism inhibits breast cancer metastasis. Cancer Res. 66 2923 PMID: 16540639

Dey et al (2009) Prostaglandin E2 couples through EP4 prostanoid receptors to induce IL-8 production in human colonic epithelial cell lines. Br.J.Pharmacol 3 475 PMID: 19175605

Kabashima et al (2002) The prostaglandin receptor EP4 suppresses colitis, mucosal damage and CD4 cell activation in the gut. J.Clin.Invest. 109 883 PMID: 11927615

Deng et al (2013) Exosome-like nanoparticles from intestinal mucosal cells carry prostaglandin E2 and suppress activation of liver NKT cells. J.Immunol. 190 3579 PMID: 23467936

If you know of a relevant reference for ONO AE3 208, please let us know.

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Keywords: ONO AE3 208, ONO AE3 208 supplier, ONOAE3208, selective, EP4, receptors, antagonists, prostanoids, PGE2, prostaglandins, high, affinity, orally, active, Prostanoid, Receptors, 3565, Tocris Bioscience

2 Citations for ONO AE3 208

Citations are publications that use Tocris products. Selected citations for ONO AE3 208 include:

David M et al (2020) The transition of M-CSF-derived human macrophages to a growth-promoting phenotype. Blood Adv 4 5460-5472 PMID: 33166408

Na-Ryum et al (2023) An airway-to-brain sensory pathway mediates influenza-induced sickness. Nature 615 660-667 PMID: 36890237

Do you know of a great paper that uses ONO AE3 208 from Tocris? Please let us know.

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