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1-(2-nitrophenyl)ethyl caged proton that releases a proton and a sulfate ion upon photolysis at 350 nm. Generates rapid acidifications down to pH 2 (pH jumps).
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 269.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.71 mL||18.57 mL||37.15 mL|
|5 mM||0.74 mL||3.71 mL||7.43 mL|
|10 mM||0.37 mL||1.86 mL||3.71 mL|
|50 mM||0.07 mL||0.37 mL||0.74 mL|
References are publications that support the biological activity of the product.
Barth and Corrie (2002) Characterization of a new caged proton capable of inducing large pH jumps. Biophys.J. 83 2864 PMID: 12414718
Abbruzzetti et al (2005) Kinetics of proton release after flash photolysis of 1-(2-Nitrophenyl)ethyl sulfate (caged sulfate) in aqueous solution. J.Am.Chem.Soc. 127 9865 PMID: 15998092
Peralvarez-Marin et al (2008) Time-resolved infrared spectroscopy of pH-induced aggregation of the Alzheimer Aβ1-28 peptide. J.Mol.Biol. 379 589 PMID: 18462754
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Keywords: NPE-caged-proton, NPE-caged-proton supplier, Caged, proton, Compounds, NPEC-caged-sulfate, Other, 3512, Tocris Bioscience
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When photolyzed Caged-proton is supposed to reduce pH; even strong 365nm UV (~1W/cm^2) for 1 second did not show any change in pH
*Tocris response - Thank you for reviewing our product. We are sorry to that this product did not perform as expected. We have been in touch with the customer to resolve this issue.
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