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NB-caged Tyrosine hydrochloride
L-Tyrosine caged with photosensitive ortho-nitrobenzyl side chain. Rapidly releases tyrosine when photolyzed (300-350 nm excitation). Is readily incorporated into E.coli proteins via inclusion in growth media.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 352.77. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.83 mL||14.17 mL||28.35 mL|
|5 mM||0.57 mL||2.83 mL||5.67 mL|
|10 mM||0.28 mL||1.42 mL||2.83 mL|
|50 mM||0.06 mL||0.28 mL||0.57 mL|
References are publications that support the biological activity of the product.
Miller et al (1998) Flash decaging of tyrosine sidechains in an ion channel. Neuron. 20 619 PMID: 9581754
Monk et al (2017) Rapid and inexpensive evaluation of nonstandard amino acid incorporation in Escherichia coli. ACS.Synth.Biol. 6 45 PMID: 27648665
Wang et al (2019) Time-resolved protein activation by proximal decaging in living systems. Nature 569 509 PMID: 31068699
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