ML 228

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Cat.No. 4565 - ML 228 | C27H21N5 | CAS No. 1357171-62-0
Description: HIF pathway activator
Chemical Name: N-([1,1'Biphenyl]-4-ylmethyl)-6-phenyl-3-(2-pyridinyl)-1,2,4-triazin-5-amine
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (2)

Biological Activity for ML 228

ML 228 is a HIF pathway activator (EC50 values are 1.23 and 1.4 μM for HRE gene reporter assay and HIF-1α nuclear translocation assay respectively); acts via chelation of iron, independently of PHD. Also exhibits > 80% inhibition of the human A3 receptor, dopamine transporter, μ receptor, hERG and 5-HT2B receptor, and rat sodium channel site 2, at a concentration of 10 μM.

Compound Libraries for ML 228

ML 228 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for ML 228

M. Wt 415.49
Formula C27H21N5
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1357171-62-0
PubChem ID 46742353
InChI Key QNRODODTMXCRKU-UHFFFAOYSA-N
Smiles C1(C2=NC(NCC4=CC=C(C5=CC=CC=C5)C=C4)=C(C3=CC=CC=C3)N=N2)=NC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ML 228

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 41.55 100
ethanol 8.31 20

Preparing Stock Solutions for ML 228

The following data is based on the product molecular weight 415.49. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.41 mL 12.03 mL 24.07 mL
5 mM 0.48 mL 2.41 mL 4.81 mL
10 mM 0.24 mL 1.2 mL 2.41 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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References for ML 228

References are publications that support the biological activity of the product.

Theriault et al (2012) Discovery of a new molecular probe ML228: an activator of the hypoxia inducible factor (HIF) pathway. Bioorg.Med.Chem.Lett. 22 76 PMID: 22172704


If you know of a relevant reference for ML 228, please let us know.

View Related Products by Product Action

View all Hypoxia Inducible Factor (HIF) Activators

Keywords: ML 228, ML 228 supplier, ML228, hypoxia, inducible, factor, HIF, pathway, activators, iron, chelators, HRE, Hypoxia, Inducible, Factors, 4565, Tocris Bioscience

Citations for ML 228

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