ML 228

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Cat.No. 4565 - ML 228 | C27H21N5 | CAS No. 1357171-62-0
Description: HIF pathway activator
Chemical Name: N-([1,1'Biphenyl]-4-ylmethyl)-6-phenyl-3-(2-pyridinyl)-1,2,4-triazin-5-amine
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

HIF pathway activator (EC50 values are 1.23 and 1.4 μM for HRE gene reporter assay and HIF-1α nuclear translocation assay respectively); acts via chelation of iron, independently of PHD. Also exhibits > 80% inhibition of the human A3 receptor, dopamine transporter, μ receptor, hERG and 5-HT2B receptor, and rat sodium channel site 2, at a concentration of 10 μM.

Compound Libraries

ML 228 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 415.49
Formula C27H21N5
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1357171-62-0
PubChem ID 46742353
InChI Key QNRODODTMXCRKU-UHFFFAOYSA-N
Smiles C1(C2=NC(NCC4=CC=C(C5=CC=CC=C5)C=C4)=C(C3=CC=CC=C3)N=N2)=NC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 41.55 100
ethanol 8.31 20

Preparing Stock Solutions

The following data is based on the product molecular weight 415.49. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.41 mL 12.03 mL 24.07 mL
5 mM 0.48 mL 2.41 mL 4.81 mL
10 mM 0.24 mL 1.2 mL 2.41 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Theriault et al (2012) Discovery of a new molecular probe ML228: an activator of the hypoxia inducible factor (HIF) pathway. Bioorg.Med.Chem.Lett. 22 76 PMID: 22172704


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View Related Products by Product Action

View all Hypoxia Inducible Factor Activators

Keywords: ML 228, supplier, ML228, hypoxia, inducible, factor, HIF, pathway, activators, iron, chelators, HRE, HIF, Tocris Bioscience

Citations for ML 228

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Angiogenesis in Cancer

Angiogenesis in Cancer Poster

Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the pathogenesis of angiogenesis in cancer, as well as some of the main angiogenesis therapeutic targets.

Pathways for ML 228

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