MIRA-1

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Cat.No. 3362 - MIRA-1 | C8H9NO4 | CAS No. 72835-26-8
Description: Restores mutant p53 activity; proapoptotic
Chemical Name: 1-[(1-Oxopropoxy)methyl]-1H-pyrrole-2,5-dione
Datasheet
Citations
Literature
Pathways

Biological Activity

Restores wild-type conformation, function and DNA binding activity to mutant p53. Induces p53 transcriptional transactivation of p21, MDM2 and PUMA, and promotes tumor cell death by apoptosis in a mutant p53-dependent manner in vitro (IC50 = 10μM).

Technical Data

M. Wt 183.16
Formula C8H9NO4
Storage Store at +4°C
CAS Number 72835-26-8
PubChem ID 227681
InChI Key YXEWPGYLMHXLPS-UHFFFAOYSA-N
Smiles O=C(N1COC(CC)=O)C=CC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 18.32 100
ethanol 18.32 100

Preparing Stock Solutions

The following data is based on the product molecular weight 183.16. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.46 mL 27.3 mL 54.6 mL
5 mM 1.09 mL 5.46 mL 10.92 mL
10 mM 0.55 mL 2.73 mL 5.46 mL
50 mM 0.11 mL 0.55 mL 1.09 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Bykov et al (2005) Reactivation of mutant p53 and induction of apoptosis in human tumor cells by maleimide analogs. J.Biol.Chem. 280 30384 PMID: 15998635

Wiman (2006) Strategies for therapeutic targeting of the p53 pathway in cancer. Cell Death Differ. 13 921 PMID: 16557267

Kumar et al (2007) p53 in breast cancer: mutation and countermeasures. Front.Biosci. 12 4168 PMID: 17485365


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View Related Products by Target

Keywords: Restores mutant p53 activity proapoptotic Transcription Factors p53

Citations for MIRA-1

Citations are publications that use Tocris products.

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Reviews

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for MIRA-1

p53

p53 Signaling Pathway

p53 signaling plays an important role in the co-ordination of the cellular response different types of stress such as DNA damage and hypoxia. The downstream signals lead to apoptosis, senescence and cell cycle arrest.

Protocols

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