JH 295

Pricing Availability   Qty
Description: Irreversible Nek2 inhibitor
Chemical Name: N-[(3Z)-3-[(2-Ethyl-4-methyl-1H-imidazol-5-yl)methylene]-2,3-dihydro-2-oxo-1H-indol-5-yl]-2-propynamide
Purity: ≥98% (HPLC)
Literature (4)

Biological Activity for JH 295

JH 295 is an irreversible Nek2 inhibitor (IC50=770 nM). Inhibits Nek2 via alkylation of residue Cys22. Displays 5-fold selectivity for Nek2 over Cdk1. Exhibits no significant effect on Aurora B or Plk1 kinases, bipolar spindle assembly or spindle assembly checkpoint. Cell permeable.

Technical Data for JH 295

M. Wt 320.35
Formula C18H16N4O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1311143-71-1
PubChem ID 53262909
Smiles O=C2NC1=CC=C(NC(C#C)=O)C=C1/C2=C/C3=C(C)N=C(CC)N3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for JH 295

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 32.03 100

Preparing Stock Solutions for JH 295

The following data is based on the product molecular weight 320.35. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.22 mL
5 mM 0.62 mL 3.12 mL 6.24 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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Product Datasheets for JH 295

Certificate of Analysis / Product Datasheet
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References for JH 295

References are publications that support the biological activity of the product.

Henise et al (2011) Irreversible Nek2 kinase inhibitors with cellular activity. J.Med.Chem. 54 4133 PMID: 21627121

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Keywords: JH 295, JH 295 supplier, JH295, Nek2, inhibitor, inhibits, irreversible, alkylation, Kinase, 4322, Tocris Bioscience

Citations for JH 295

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Literature in this Area

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