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Description: Fluorescent probe for labeling mutant FKBP12F36V proteins
Chemical Name: (R)-1-(2-(2-((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)amino)-2-oxoethoxy)phenyl)-3-(3,4-dimethoxyphenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
Purity: ≥95% (HPLC)
Literature (3)

Biological Activity for dTAG-Fluorescein

dTAG-Fluorescein is a fluorescent probe for labeling FKBP12F36V fusion proteins. Comprises a high-affinity ligand for FKBP12F36V mutant protein (Kd in the subnanomolar range and 1,000-fold selectivity for the F36V mutant of FKBP12 over wild-type) coupled to 5-amino fluorescein. dTAG-Fluorescein can fluorescently label FKBP12F36V fusion proteins expressed at different levels in a variety of cell lines.

Optical Data for dTAG-Fluorescein

dTAG-Fluorescein Dye Spectra
Emission Color Green
λabs 493 nm
λem 514 nm
Extinction Coefficient (ε) 68800 M-1cm-1
Closest Laser line 488 nm
Application Flow cytometry, Confocal microscopy

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Technical Data for dTAG-Fluorescein

M. Wt 1023.1
Formula C58H58N2O15
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NC4=CC(C(O5)=O)=C(C=C4)C65C7=C(C=C(C=C7)O)OC8=C6C=CC(O)=C8)=O)CCC9=CC(OC)=C(C=C9)OC)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for dTAG-Fluorescein

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 10.23 10

Preparing Stock Solutions for dTAG-Fluorescein

The following data is based on the product molecular weight 1023.1. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 9.77 mL 48.87 mL 97.74 mL
0.5 mM 1.95 mL 9.77 mL 19.55 mL
1 mM 0.98 mL 4.89 mL 9.77 mL
5 mM 0.2 mL 0.98 mL 1.95 mL

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Product Datasheets for dTAG-Fluorescein

References for dTAG-Fluorescein

References are publications that support the biological activity of the product.

Nabet et al (2018) The dTAG system for immediate and target-specific protein degradation. Nat.Chem.Biol. 14 431 PMID: 29581585

Marks et al (2004) A general approach for chemical labeling and rapid, spatially controlled protein inactivation. Proc.Natl.Acad.Sci.U.S.A. 101 9982 PMID: 15218100

If you know of a relevant reference for dTAG-Fluorescein, please let us know.

Keywords: dTAG-Fluorescein, dTAG-Fluorescein supplier, chemical, labeling, probe, FKBP12(F36V), dTAG, fluorescein, fluorescence, microscopy, TAG, Degradation, Platform, Other, Fluorescent, Probes, 7892, Tocris Bioscience

Citations for dTAG-Fluorescein

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Targeted Protein Degradation Research Product Guide

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

  • Active Degraders
  • TAG Degradation Platform
  • Degrader Building Blocks
  • Ubiquitin-Proteasome System Proteins
  • Assays for Protein Degradation
Targeted Protein Degradation Poster

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia

Validating Targets for TPD Using dTAG Poster

Validating Targets for TPD Using dTAG Poster

The dTAG platform offers a generalizable strategy to degrade, in principle, any intracellular protein of interest (POI) and is a useful strategy for exploration and validation of targets. This poster presents a workflow solution for target validation, from custom TAG knock-in cell-lines for your POI, different dTAG Degraders to knockdown you POI, to automated assays for protein degradation using Simple WesternTM instruments. Data are also presented on the use of an antibody that recognizes the TAG domain (FKBP12F36V) for detection of degradation. Presented at TPD Europe, March 2022, London, UK.