CRF (human, rat)

Pricing Availability Delivery Time Qty
Cat.No. 1151 - CRF (human, rat) | Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2 | CAS No. 86784-80-7
Description: Stimulates ACTH release
Alternative Names: Corticotropin-Releasing Factor (human, rat)
Datasheet
Citations (7)
Literature

Biological Activity

Endogenous peptide agonist for the CRF receptor (Ki values are 11, 44 and 38 nM for hCRF1, rCRF2a and mCRF2b respectively). Stimulates the synthesis and release of ACTH from the anterior pituitary.

Licensing Information

Sold with the permission of the SALK Institute

Technical Data

M. Wt 4758
Formula C208H344N60O63S2
Sequence SEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEII

(Modifications: Ile-41 = C-terminal amide)

Storage Desiccate at -20°C
CAS Number 86784-80-7
PubChem ID 16132357
InChI Key VXFVFWFSJFSXHN-FAUHKOHMSA-N
Smiles [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

SolubilitySoluble to 1.10 mg/ml in water

Preparing Stock Solutions

The following data is based on the product molecular weight 4758. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.21 mL 1.05 mL 2.1 mL
5 mM 0.04 mL 0.21 mL 0.42 mL
10 mM 0.02 mL 0.11 mL 0.21 mL
50 mM 0 mL 0.02 mL 0.04 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Perrin and Vale (1999) Corticotropin releasing factor receptors and their ligand family. Ann.N.Y.Acad.Sci. 885 312 PMID: 10816663

Rivier et al (1986) Mediation by corticotropin releasing factor (CRF) of adenohypophysial hormone secretion. Annu.Rev.Physiol. 48 475 PMID: 2871808

Souza et al (1984) Corticotropin-releasing factor receptors in rat forebrain: autoradiographic identification. Science 224 1449 PMID: 6328656

Tache et al (1983) Inhibition of gastric acid secretion in rats by intracerebral injection of corticotropin-releasing factor. Science 222 935 PMID: 6415815

Perrin et al (1999) Comparison of an agonist, urocortin, and an antagonist, astressin, as radioligands for characterization of corticotropin-releasing factor receptors. J.Pharmacol.Exp.Ther. 288 729 PMID: 9918582

Vale et al (1981) Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and β-endorphin. Science 213 1394 PMID: 6267699


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Keywords: Stimulates ACTH release Corticotropin-Releasing Factor Non-Selective CRF Receptors agonists Corticotropin-Releasing Factor (human, rat) Non-selective CRF

7 Citations for CRF (human, rat)

Citations are publications that use Tocris products. Selected citations for CRF (human, rat) include:

Land et al (2008) The dysphoric component of stress is encoded by activation of the dynorphin kappa-opioid system. J Neurosci 28 407 PMID: 18184783

Dermitzaki et al (2007) Corticotropin-releasing factor (CRF) and the urocortins differentially regulate catecholamine secretion in human and rat adrenals, in a CRF receptor type-specific manner. Endocrinology 148 1524 PMID: 17194738

Song et al (2016) Systemic pro-inflammatory response facilitates the development of cerebral edema during short hypoxia. J Neurosci 13 63 PMID: 26968975

Klampfl et al (2016) CRF-R1 activation in the anterior-dorsal BNST induces maternal neglect in lactating rats via an HPA axis-independent central mechanism. J Neuroinflammation 64 89 PMID: 26630389

Gray et al (2015) Corticotropin-releasing hormone drives anandamide hydrolysis in the amygdala to promote anxiety. J Neurosci 35 3879 PMID: 25740517

Navarro et al (2015) Orexin-corticotropin-releasing factor receptor heteromers in the ventral tegmental area as targets for cocaine. Psychoneuroendocrinology 35 6639 PMID: 25926444

Lowery-Gionta et al (2012) Corticotropin releasing factor signaling in the central amygdala is recruited during binge-like ethanol consumption in C57BL/6J mice. J Neurosci 32 3405 PMID: 22399763


Do you know of a great paper that uses CRF (human, rat) from Tocris? If so please let us know.

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Pathways for CRF (human, rat)

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