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Submit Review7α,25-Dihydroxycholesterol
Description: Highly potent GPR183 (EBI2) agonist
Chemical Name: (3β,7α)-Cholest-5-ene-3,7,25-triol
Purity: ≥98% (HPLC)
Biological Activity
Highly potent GPR183 (EBI2) agonist (EC50 = 140 pM) and putative natural ligand for GPR183. Regulates migration of B and T cells expressing GPR183 in vivo. Desensitizes B cells and reduces their movement to splenic follicles.
Technical Data
| M. Wt | 418.65 |
| Formula | C27H46O3 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 64907-22-8 |
| PubChem ID | 11954197 |
| InChI Key | BQMSKLCEWBSPPY-IKVTXIKFSA-N |
| Smiles | O[C@H](C1)CC[C@@]2(C)C1=C[C@@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)(O)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.19 | 10 with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 418.65. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 23.89 mL | 119.43 mL | 238.86 mL |
| 0.5 mM | 4.78 mL | 23.89 mL | 47.77 mL |
| 1 mM | 2.39 mL | 11.94 mL | 23.89 mL |
| 5 mM | 0.48 mL | 2.39 mL | 4.78 mL |
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References
References are publications that support the biological activity of the product.
Liu et al (2011) Oxysterols direct B-cell migration through EBI2. Nature. 475 519 PMID: 21796211
Hannedouche et al (2011) Oxysterols direct immune cell migration via EBI2. Nature. 475 524 PMID: 21796212
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Keywords: 7alpha,25-Dihydroxycholesterol, 7alpha,25-Dihydroxycholesterol supplier, 7a,25-Dihydroxycholesterol, GPR183, EBI2, B, cell, T, migration, densensitizer, active, in, vivo, Epstein, Barr, virus, induced, G, protein, coupled, receptor, 2, infection, spleen, Orphan, 7-TM, Receptors, 6277, Tocris Bioscience
Citations for 7α,25-Dihydroxycholesterol
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