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7α,25-Dihydroxycholesterol

Cat. No. 6277 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Cat.No. 6277 - 7alpha,25-Dihydroxycholesterol | C27H46O3 | CAS No. 64907-22-8
Description: Highly potent GPR183 (EBI2) agonist
Chemical Name: (3β,7α)-Cholest-5-ene-3,7,25-triol
Purity: ≥98% (HPLC)
Datasheet
Citations
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Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for 7α,25-Dihydroxycholesterol

7α,25-Dihydroxycholesterol is a highly potent GPR183 (EBI2) agonist (EC50 = 140 pM) and putative natural ligand for GPR183. Regulates migration of B and T cells expressing GPR183 in vivo. Desensitizes B cells and reduces their movement to splenic follicles.

Technical Data for 7α,25-Dihydroxycholesterol

M. Wt 418.65
Formula C27H46O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 64907-22-8
PubChem ID 11954197
InChI Key BQMSKLCEWBSPPY-IKVTXIKFSA-N
Smiles O[C@H](C1)CC[C@@]2(C)C1=C[C@@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)(O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 7α,25-Dihydroxycholesterol

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.19 10 with gentle warming

Preparing Stock Solutions for 7α,25-Dihydroxycholesterol

The following data is based on the product molecular weight 418.65. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 23.89 mL 119.43 mL 238.86 mL
0.5 mM 4.78 mL 23.89 mL 47.77 mL
1 mM 2.39 mL 11.94 mL 23.89 mL
5 mM 0.48 mL 2.39 mL 4.78 mL

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Product Datasheets for 7α,25-Dihydroxycholesterol

Certificate of Analysis / Product Datasheet
Safety Datasheet

References for 7α,25-Dihydroxycholesterol

References are publications that support the biological activity of the product.

Liu et al (2011) Oxysterols direct B-cell migration through EBI2. Nature. 475 519 PMID: 21796211

Hannedouche et al (2011) Oxysterols direct immune cell migration via EBI2. Nature. 475 524 PMID: 21796212

If you know of a relevant reference for 7α,25-Dihydroxycholesterol, please let us know.

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Keywords: 7alpha,25-Dihydroxycholesterol, 7alpha,25-Dihydroxycholesterol supplier, 7a,25-Dihydroxycholesterol, GPR183, EBI2, B, cell, T, migration, densensitizer, active, in, vivo, Epstein, Barr, virus, induced, G, protein, coupled, receptor, 2, infection, spleen, Orphan, 7-TM, Receptors, 6277, Tocris Bioscience

Citations for 7α,25-Dihydroxycholesterol

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