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Description: Muscarinic M3 antagonist
Chemical Name: 1,1-Dimethyl-4-diphenylacetoxypiperidinium iodide
Purity: ≥99% (HPLC)
Biological Activity for 4-DAMP
4-DAMP is an antagonist at the M3 cholinergic receptor. [3H]-4-DAMP selectively labels M1 and M3 receptors.
Technical Data for 4-DAMP
| M. Wt | 451.33 |
| Formula | C21H26INO2 |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 1952-15-4 |
| PubChem ID | 3014059 |
| InChI Key | WWJHRSCUAQPFQO-UHFFFAOYSA-M |
| Smiles | [I-].C[N+]1(C)CCC(CC1)OC(=O)C(C1=CC=CC=C1)C1=CC=CC=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for 4-DAMP
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.28 | 25 |
Preparing Stock Solutions for 4-DAMP
The following data is based on the product molecular weight 451.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.25 mM | 8.86 mL | 44.31 mL | 88.63 mL |
| 1.25 mM | 1.77 mL | 8.86 mL | 17.73 mL |
| 2.5 mM | 0.89 mL | 4.43 mL | 8.86 mL |
| 12.5 mM | 0.18 mL | 0.89 mL | 1.77 mL |
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Product Datasheets for 4-DAMP
References for 4-DAMP
References are publications that support the biological activity of the product.
Barlow and Shepherd (1986) A further search for selective antagonists at M2- muscarinic receptors. Br.J.Pharmacol. 89 837 PMID: 3814912
Michel et al (1989) Direct labelling of rat M3-muscarinic receptors by [3H]4-DAMP. Eur.J.Pharmacol. 166 459 PMID: 2806372
If you know of a relevant reference for 4-DAMP, please let us know.
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Keywords: 4-DAMP, 4-DAMP supplier, Muscarinic, M3, antagonists, Receptors, Acetylcholine, Ach, 0482, Tocris Bioscience
16 Citations for 4-DAMP
Citations are publications that use Tocris products. Selected citations for 4-DAMP include:
Kajiya et al (2012) Muscarinic type 3 receptor induces cytoprotective signaling in salivary gland cells through epidermal growth factor receptor transactivation. J Neurosci 82 115 PMID: 22511543
Solanki et al (2007) Cholinergic innervation of the guinea-pig isolated vas deferens. Naunyn Schmiedebergs Arch Pharmacol 376 265 PMID: 17992517
Hodges et al (2012) Signaling pathways used by EGF to stimulate conjunctival goblet cell secretion. Exp Eye Res 103 99 PMID: 22975404
Clancy et al (2007) Coregulation of natively expressed pertussis toxin-sensitive muscarinic receptors with G-protein-activated potassium channels. Physiol Rep 27 6388 PMID: 17567799
Emmerson et al (2017) SOX2 regulates acinar cell development in the salivary gland. Elife 6 PMID: 28623666
Matsumoto et al (2010) Differential roles of M2 and M3 muscarinic receptor subtypes in modulation of bladder afferent activity in rats. Urology 75 862 PMID: 20156651
Wallace et al (2015) Regulation of nerve-evoked contractions of rabbit vas deferens by acetylcholine. Microvasc Res 3 PMID: 26359240
Chen et al (2012) Mechanism of all-trans-retinal toxicity with implications for stargardt disease and age-related macular degeneration. J Biol Chem 287 5059 PMID: 22184108
Howell et al (2005) In vivo antimuscarinic actions of the third generation antihistaminergic agent, deslorat. BMC Pharmacol 5 13 PMID: 16109168
Nedvetsky et al (2014) Parasympathetic innervation regulates tubulogenesis in the developing salivary gland. Dev Cell 30 449 PMID: 25158854
Placeres-Uray et al (2014) M2 Muscarinic acetylcholine receptor modulates rat airway smooth muscle cell proliferation. World Allergy Organ J 6 22 PMID: 24377382
Pacini et al (2014) M2muscarinic receptors inhibit cell proliferation and migration in urothelial bladder cancer cells. Cancer Biol Ther 15 1489 PMID: 25482946
Mans et al (2014) An acetylcholinesterase inhibitor, eserine, induces long-term depression at CA3-CA1 synapses in the hippocampus of adult rats. J Neurophysiol 112 2388 PMID: 25143547
Jahchan et al (2013) A drug repositioning approach identifies tricyclic antidepressants as inhibitors of small cell lung cancer and other neuroendocrine tumors. Cancer Discov 3 1364 PMID: 24078773
Kitazawa et al (2009) M3 muscarinic receptors mediate positive inotropic responses in mouse atria: a study with muscarinic receptor knockout mice. Mol Pharmacol 330 487 PMID: 19429792
Fonseca et al (2015) Diadenosine tetraphosphate contributes to CB-induced tear secretion. Purinergic Signal 11 87 PMID: 25398705
Do you know of a great paper that uses 4-DAMP from Tocris? Please let us know.
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