N-Acetylcysteine

Name: N -Acetylcysteine
Brand: Tocris Bioscience
SKU: 7874
Rating: 5 (1 reviews)

Cat. No. 7874 1 条评论提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Pricing	Availability		Qty

Save 15% on Select RUO Reagents. See Details.

说明： Glutathione (GSH) precursor; maintains stem cell function in culture.

化学名： (2R)-2-Acetamido-3-sulfanylpropanoic acid

纯度： ≥98% (HPLC)

说明书

引用文献

生物活性 Scientific Data 技术数据溶解性计算器说明书参考文献

生物活性 for N-Acetylcysteine

N-Acetylcysteine is a cell-permeable antioxidant and a precursor of reduced glutathione (GSH) (Cat. No. 5219), with anti-inflammatory, mucolytic, and antiviral activities. N-Acetylcysteine can be used in organoid culture media for non-small cell lung and colorectal cancer organoids, expansion media for mouse/human liver and pancreas 3D organoids and LWRN media for colonic 3D organoids from human biopsies. It is a component of S7 medium in the optimized protocol for generation of functional stem cell-derived islets (SC-islets). N-Acetylcysteine can be orally administered.

Scientific Data

View Larger

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Lung Organoids. Adult stem cells isolated from human lung biopsy tissue were embedded and then cultured for 20-60 days in lung organoid expansion medium, which includes N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), SB 202190 (Catalog # 1264), Nicotinamide (Catalog # 4106), along with other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various lung cell types. Lung organoids were stained with (A) anti-Cytokeratin 5 (KRT5) (green) and with anti-p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (B) Anti-Podoplanin (PDPN) (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and anti- p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (C, D) Anti-MUC5AC (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and anti-SOX2 (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).

View Larger

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Liver Organoids. Adult stem cell-derived liver organoids were cultured using N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), Forskolin (Catalog # 1099), along with other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes N-Acetylcysteine, HEPES (Catalog # 3173), Nicotinamide (Catalog # 4106), A 83-01 (Catalog # 2939), along with other reagents. Differentiated human liver organoids were stained using a (A) Human Serum Albumin Antibody (Catalog # MAB1455; red) and a (B) Human HNF-3beta /FoxA2 Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).

View Larger

Application of N-Acetylcysteine in Culture and Characterization of iPSC-derived Human Intestinal Organoids. iPSC-derived human intestinal organoids were cultured using N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Human/Mouse/Rat Vimentin Antibody (Catalog # MAB2105; green) and a Human/Mouse Desmin Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Human/Mouse E-Cadherin Antibody (Catalog # AF748; green), a Human MUC2 Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).

View Larger

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Human Descending Colon Organoids. Adult stem cells isolated from human descending colon were embedded and then cultured for 30 days in intestinal organoid culture medium, which includes N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with Anti-MUC2 (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained for E-Cadherin (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue). (B) Organoids were fixed and stained with Human Chromogranin A Antibody(Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained for E-Cadherin (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue).

技术数据 for N-Acetylcysteine

分子量	163.2
公式	C₅H₉NO₃S
储存	Store at -20°C
纯度	≥98% (HPLC)
CAS Number	616-91-1
PubChem ID	12035
InChI Key	PWKSKIMOESPYIA-BYPYZUCNSA-N
Smiles	[C@@H](NC(C)=O)(C(O)=O)CS

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for N-Acetylcysteine

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
	DMSO	8.16	50
	water	8.16	50

制备储备液 for N-Acetylcysteine

以下数据基于产品分子量 163.2。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.5 mM	12.25 mL	61.27 mL	122.55 mL
2.5 mM	2.45 mL	12.25 mL	24.51 mL
5 mM	1.23 mL	6.13 mL	12.25 mL
25 mM	0.25 mL	1.23 mL	2.45 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt* g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1

Volume 1

Concentration 2

Volume 2

产品说明书 for N-Acetylcysteine

分析证书/产品说明书

化学品安全技术说明书

参考文献 for N-Acetylcysteine

参考文献是支持产品生物活性的出版物。

Cattaneo et al (2019) Tumor organoid-T-cell coculture systems. Nat.Protoc. 15 15 PMID: 31853056

Broutier et al (2016) Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat.Protoc. 11 1724 PMID: 27560176

Balboa et al (2022) Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. Nat.Biotechnol. 40 1042 PMID: 35241836

Moiseeva et al (2023) Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration. Nature 613 169 PMID: 36544018

Geiler et al (2009) N-acetyl-L-cysteine (NAC) inhibits virus replication and expression of pro-inflammatory molecules in A549 cells infected with highly pathogenic H5N1 influenza A virus. Biochem.Pharmacol. 79 413 PMID: 19732754

If you know of a relevant reference for N-Acetylcysteine, please let us know.

按标靶查看相关产品

关键词: N-Acetylcysteine, N-Acetylcysteine supplier, cell, permeable, antioxidant, glutathione, precursor, GSH, organoids, stem, cells, differentiation, mucolytic, antiviral, anti-inflammatory, NAC, nacetylcysteine, Antioxidants, Neural, Stem, Cells, Organoids, Cancer, ESCs, and, iPSC, Mesenchymal, Other, Differentiation, Products, Influenza, Viruses, 7874, Tocris Bioscience

篇 N-Acetylcysteine 的引用文献

引用文献是使用了 Tocris 产品的出版物。

目前没有 N-Acetylcysteine 的引用文献。您是否知道使用了 Tocris N-Acetylcysteine 的优秀论文？请告知我们.