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Flutax 2

Cat. No. 6254 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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6254 has been discontinued.

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说明： Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions
化学名： (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(2',7'-difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine
纯度： ≥90% (HPLC)
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生物活性 for Flutax 2

Key information: Flutax 2 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.

Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions.

Application: fluorescence microscopy.

Properties and Photophysical Data: The fluorescent label on Flutax 2 is attached by derivatization of the 7-β-hydroxy group of native paclitaxel which permits selective binding of the probe to microtubules. Flutax 2 binds microtubules with high affinity (Ka ~ 107M-1). Flutax 2 binds to α, β tubulin dimers and stabilizes them. Flutax 2 is soluble and pH insensitive and is more photostable than Flutax 1 (Cat. No. 2226). Excitation and emission maxima (λ) are 496 nm and 526 nm, respectively.

Scientific Data

Fluorescence Imaging Application of Flutax 2 for labeling HeLa cells View Larger

Flutax 2 labeled HeLa cells. Flutax 2 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 2 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 2 staining is not retained at all after fixation (photobleach). Like Flutax 1, Flutax 2 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good signal.

Optical Data for Flutax 2

Flutax 2 Dye Spectra
λabs 496 nm
λem 526 nm
Extinction Coefficient (ε) 80000 M-1cm-1
Quantum Yield (φ) 0.9
Application Fluorescence Microscopy

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技术数据 for Flutax 2

分子量 1319.28
公式 C71H64F2N2O21
储存 Store at -20°C
纯度 ≥90% (HPLC)
CAS Number 301844-13-3
PubChem ID 44571006
InChI Key UTOYCOKRJWZPNT-SQNCZLKBSA-N
Smiles O=C1[C@@]2(C)C([C@@]3(OC(C)=O)[C@H](OC3)C[C@@H]2OC([C@H](C)NC(C4=CC=C(C5=C(C=C6F)C(OC7=C5C=C(F)C(O)=C7)=CC6=O)C(C(O)=O)=C4)=O)=O)[C@H](OC(C8=CC=CC=C8)=O)[C@@]9(O)C(C)(C)C([C@H]1OC(C)=O)=C(C)[C@@H](OC([C@H](O)[C@H](C%10=CC=CC=C%10)NC(C%11=CC=CC=C%11)=O)=O)C9

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

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参考文献 for Flutax 2

参考文献是支持产品生物活性的出版物。

Lillo et al (2002) Location and properties of the Tax. binding center in microtubules: a picosecond laser study with fluorescent taxoids. Biochemistry. 41 12436 PMID: 12369834

Diaz et al (2000) Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site. J.Biol.Chem. 275 26265 PMID: 10818101

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关键词: Flutax 2, Flutax 2 supplier, paclitaxel, Flutax2, Taxol, fluorescent, dye, labeled, labelled, microtubules, tubulin, oregon, green, Microtubules, Mitosis, Organelle, and, Cell, Structure, Stains, Microtubule, Probes, 6254, Tocris Bioscience

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Fluorescent Dyes and Probes Research Product Guide

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