ClariTSA™ Fluorophore Kit 650

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说明: Signal amplification kit for use in ISH, ICC and IHC
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生物活性 for ClariTSA™ Fluorophore Kit 650

Please note: this product was formerly known as TSA Vivid Fluorophore Kit 650.

ClariTSA™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. ClariTSA reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.

The basis for signal amplification comes from the specific and high-density deposition of ClariTSA dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).

Excitation maximum = 654 nm; emission maximum = 668 nm

Please see the product datasheet or protocol for information on product use.

ClariTSA™ is a trademark of Bio-Techne Corp.

Scientific Data

Application of ClariTSA™ Fluorophore Kit 650 in RNAscope™ Multiplex Fluorescent v2 Assay. RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and ClariTSA™ Fluorophore Kit 650 (right). Dye used at 1:1500 dilution. Marker shown is UBC in white. Nuclei are counterstained with DAPI (blue, Catalog # 5748, Tocris).

Optical Data for ClariTSA™ Fluorophore Kit 650

Spectral data for ClariTSA Fluorophore Kit 650 Emission Color Red
λabs 654 nm
λem 668 nm

技术数据 for ClariTSA™ Fluorophore Kit 650

储存 Store at -20°C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

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关键词: ClariTSA Fluorophore Kit 650, ClariTSA Fluorophore Kit 650 supplier, RNAscope, tyramide, signal, amplification, TSA, Fluorescent, fluorescence, assay, Catalyzed, Reporter, Deposition, CARD, horseradish, peroxidase, HRP, kits, multiplexing, Vivid, Tyramide, Signaling, Amplification, (TSA), 7527, Tocris Bioscience

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including Janelia Fluor® Dyes
  • Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
  • Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
  • Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals
  • Tissue Clearing Kits and Reagents