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NucleoLIVE™ Non-Toxic Dye

Cat. No. 8935 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Ready-to-use fluorescent nuclear stain for live cell imaging, cell toxicity, viability and morphology of 2D and 3D cell cultures
纯度： ≥90% (HPLC)
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实验方案 (1)
生物活性 Scientific Data 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for NucleoLIVE™ Non-Toxic Dye

NucleoLIVE™ Non-Toxic Dye is a ready-to-use fluorescent nuclear stain for live cell imaging. NucleoLIVE™ Non-Toxic Dye is highly suited for long term 2D and 3D cell organoids imaging. Able to multiplex with Live-dead stain for multiplexed phenotypic imaging. NucleoLIVE™ Non-Toxic Dye is suitable for monitoring precise tracking of cell proliferation, differentiation, and responses to stimuli over time. Allow wash-free imaging. NucleoLIVE™ Non-Toxic Dye is provided as 50 µL of 1000x concentrate in DMSO. Excitation and emission maxima (λ) are 568 nM and 590 nM, respectively.

Please refer to this protocol for further information on how to use this product.

NucleoLIVE™ is a trademark of Saguaro Biosciences.

Scientific Data

Live-cell Imaging View Larger

NucleoLIVE™ Non-Toxic Dye Application. NucleoLIVE™ staining of 3D brain organoids under hypoxic conditions. Live imaging shows deep penetration and uniform nuclear staining (1:500, red), demonstrating suitability for complex 3D models.

Live-cell Imaging Ready-to-use fluorescent nuclear stain for live cell imaging, cell toxicity, viability and morphology of 2D and 3D cell cultures View Larger

NucleoLIVE™ Non-Toxic Dye Application. MCF-7 cells co-stained with NucleoLIVE™ (1:500) and Hoechst 33342 following 4-hour incubation. Image captured at 40× magnification, showing nuclear co-localization and live-cell compatibility.

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NucleoLIVE™ Non-Toxic Dye Application. Comparison of proliferation rates in MCF-7 cells: stable nuclear GFP reporter line versus NucleoLIVE™ staining at various concentrations (1×: 1:1,000; 0.5×: 1:2,000; 0.25×: 1:4,000). Controls include untreated (“Medium”) and 0.1% DMSO. Assays performed on an Incucyte Live-Cell Analysis System by Prof. John Hawse, Mayo Clinic.

许可信息

Sold under license from Saguaro Biosciences

Optical Data for NucleoLIVE™ Non-Toxic Dye

NucleoLIVE™ Non-Toxic Dye Dye Spectra
λabs 568 nm
λem 590 nm
Cell Permeable Yes

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for NucleoLIVE™ Non-Toxic Dye

储存 Store at -20°C
纯度 ≥90% (HPLC)

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for NucleoLIVE™ Non-Toxic Dye

溶解性 Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x)

产品说明书 for NucleoLIVE™ Non-Toxic Dye

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参考文献 for NucleoLIVE™ Non-Toxic Dye

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NucleoLIVE™ Non-Toxic Dye 实验方案

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