MortaLIVE™ Non-Toxic Dye

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说明: Ready-to-use fluorescent live-dead cell stain for long term live cell imaging
纯度: ≥90% (HPLC)
说明书
引用文献
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实验方案 (1)

生物活性 for MortaLIVE™ Non-Toxic Dye

MortaLIVE™ Non-Toxic Dye is a ready-to-use fluorescent live-dead cell stain for long term live cell imaging. Can be used for toxicity assays, tracking cell viability and cytotoxicity in real time by cell imaging. Excitation and emission maxima (λ) are 488 nM and 640 nM, respectively. MortaLIVE™ Non-Toxic Dye is provided as 50 μL of 1000x concentrate in DMSO.

Please refer to this protocol for further information on how to use this product.

MortaLIVE™ is a trademark of Saguaro Biosciences.

Scientific Data

Live-cell Imaging View Larger

MortaLIVE™ Non-Toxic Dye Application. Live-cell fluorescence imaging of heart organoids stained with MortaLIVE™ and NucleoLIVE™ for real-time quantification of cytotoxicity under hypoxia (top row, 4 h) or sham (bottom row, 3 h) conditions. Left column shows MortaLIVE™ staining alone; right column shows multiplexed staining with both MortaLIVE™ and NucleoLIVE™. Live cells are shown in purple, dead cells in green, and nuclei in red. Images acquired using a Zeiss Airyscan 880 confocal microscope (40× oil objective, 20 z-stacks at 1 µm step size).

Live-cell Imaging Ready-to-use fluorescent live-dead cell stain for long term live cell imaging View Larger

MortaLIVE™ Non-Toxic Dye Application. Live-cell fluorescence imaging of MCF-7 cells stained with MortaLIVE™ and NucleoLIVE™ after 24 hours under compound-treated conditions. Nuclear channel not shown.

Live-cell Imaging View Larger

MortaLIVE™ Non-Toxic Dye Application. Live-cell fluorescence imaging of heart organoids stained with MortaLIVE™ for real-time quantification of cytotoxicity under hypoxia (top row, 4 h) or sham (bottom row, 3 h) conditions. Images acquired using a Nikon-Crest spinning disk confocal microscope (20× air objective, 20 z-stacks at 5 µm step size). Left column shows live cells (red); right column shows dead cells (green).

MortaLIVE™ Non-Toxic Dye Application. Animated graph showing whole field-of-view fluorescence intensity of live and dead cell signals in MCF-7 cells stained with MortaLIVE™ following PFA treatment. MortaLIVE™ enables simultaneous detection of live and dead cells using a single dye, with distinct fluorescence signals at different wavelengths. The animation quantifies dynamic changes in cell viability over time.

许可信息

Sold under license from Saguaro Biosciences

Optical Data for MortaLIVE™ Non-Toxic Dye

MortaLIVE™ Non-Toxic Dye Dye Spectra
λabs 488 nm
λem 640 nm
Cell Permeable Yes

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for MortaLIVE™ Non-Toxic Dye

储存 Store at -20°C
纯度 ≥90% (HPLC)

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for MortaLIVE™ Non-Toxic Dye

溶解性 Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x)

产品说明书 for MortaLIVE™ Non-Toxic Dye

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参考文献 for MortaLIVE™ Non-Toxic Dye

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MortaLIVE™ Non-Toxic Dye 实验方案

以下实验方案包含使用 MortaLIVE™ Non-Toxic Dye(目录编号 8936)的更多信息。