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Submit ReviewJanelia Fluor® 635, BromoCatch™ Ligand 最新
说明： Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
化学名： 4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
纯度： ≥95% (HPLC)
生物活性
Janelia Fluor® 635, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 635, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 635 dye, delivering bright, red-shifted signal with minimal background fluorescence. Janelia Fluor® 635, BromoCatch™ Ligand enables nuclear-targeted labeling of fusion constructs such as H2B-BromoCatch, and shows no off-target activity in wild-type or untransfected cells. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 635, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.
Please refer to this protocol for further information on how to use this product.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
This product is part of an early adopter program.
BromoCatch™ is a trademark of Bio-Techne Corp.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee
Optical Data for Janelia Fluor® 635, BromoCatch™ Ligand
| λabs | 646 nm |
|---|---|
| λem | 663 nm |
| Cell Permeable | Yes |
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技术数据
| 分子量 | 1152.4 |
| 公式 | C61H67F2N9O8SSi |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| InChI Key | KUWXUURGPXZQEI-RYOPHROTSA-N |
| Smiles | CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCNC(C4=CC=C(C(C5=C6C([Si](C)(C7=CC(N8CC(C8)F)=CC=C57)C)=C/C(C=C6)=[N+]9CC(C/9)F)=C4)C([O-])=O)=O)=O)CC)C%10=NN=C(C)N2%10 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 11.52 | 10 |
制备储备液
以下数据基于产品分子量 1152.4。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8.68 mL | 43.39 mL | 86.78 mL |
| 0.5 mM | 1.74 mL | 8.68 mL | 17.36 mL |
| 1 mM | 0.87 mL | 4.34 mL | 8.68 mL |
| 5 mM | 0.17 mL | 0.87 mL | 1.74 mL |
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产品说明书
参考文献
参考文献是支持产品生物活性的出版物。
Rodriguez-Rios et al (2025) BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. biorxiv
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