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Submit ReviewJanelia Fluor® 549, BromoCatch™ Ligand 最新
说明： Fluorogenic Janelia Fluor® 549 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
化学名： 4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)benzoate
纯度： ≥90% (HPLC)
生物活性
Janelia Fluor® 549, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 549, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 549 dye, delivering bright signal with minimal background fluorescence. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 549, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.
Please refer to this protocol for further information on how to use this product.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
This product is part of an early adopter program.
BromoCatch™ is a trademark of Bio-Techne Corp.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee
技术数据
| 分子量 | 1074.27 |
| 公式 | C59H63N9O9S |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| InChI Key | KPBISVQUOTWWDN-GFPQFKIWSA-N |
| Smiles | CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCNC(C4=CC=C(C(C5=C6C(OC7=CC(N8CCC8)=CC=C57)=C/C(C=C6)=[N+]9CCC/9)=C4)C([O-])=O)=O)=O)CC)C%10=NN=C(C)N2%10 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 10.74 | 10 |
制备储备液
以下数据基于产品分子量 1074.27。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 9.31 mL | 46.54 mL | 93.09 mL |
| 0.5 mM | 1.86 mL | 9.31 mL | 18.62 mL |
| 1 mM | 0.93 mL | 4.65 mL | 9.31 mL |
| 5 mM | 0.19 mL | 0.93 mL | 1.86 mL |
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产品说明书
参考文献
参考文献是支持产品生物活性的出版物。
Rodriguez-Rios et al (2025) BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. biorxiv
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关键词: Janelia Fluor® 549, BromoCatch™ Ligand, Janelia Fluor® 549, BromoCatch™ Ligand supplier, Janelia, Fluor549-bromocatch-ligand, JF549, fluorescent, fluorogenic, cell, permeable, real-time, microscopy, live, imaging, BromoCatch-tag, super-resolution, SRM, BromoCatch, Platform, Self-labeling, Tags, 8942, Tocris Bioscience
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