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Biotin BromoCatch™ Ligand

Cat. No. 8939 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： A biotinylated covalent ligand for BromoCatch-tagged proteins, enabling selective and irreversible biotin labeling for pull-down and detection applications in cell lysates.
化学名： N-((17R)-17-((6S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-16-oxo-3,6,9,12-tetraoxa-15-azanonadecyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
纯度： ≥95% (HPLC)
说明书
引用文献
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生物活性 技术数据 说明书 参考文献

生物活性 for Biotin BromoCatch™ Ligand

Biotin BromoCatch™ Ligand is a covalent probe designed for the selective labeling and enrichment of proteins tagged with the BromoCatch™ self-labeling tag. Based on a para-acrylamide-functionalized bump-JQ1 scaffold, the BromoCatch™ ligand, enables irreversible and highly specific covalent attachment to the engineered cysteine residue in the BromoCatch-tagged protein. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. This probe demonstrates exceptional target selectivity, confirmed via intact protein mass spectrometry and western blotting, and shows no background labeling in untransfected or wild-type samples even at micromolar concentrations. Biotin BromoCatch™ Ligand expands the utility of the BromoCatch™ platform for biochemical workflows including proteomics, immunoprecipitation, and interaction studies.

Please refer to this protocol for further information on how to use this product.

BromoCatch™ is a trademark of Bio-Techne Corp.

许可信息

Sold under exclusive licence from the University of Dundee

技术数据 for Biotin BromoCatch™ Ligand

分子量 908.15
公式 C44H61N9O8S2
储存 Store at -20°C
纯度 ≥95% (HPLC)
InChI Key BZQKBFKRTBEEFV-HQANLXDBSA-N
Smiles CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCOCCNC(CCCC[C@H]4[C@](NC(N5)=O)([H])[C@]5([H])CS4)=O)=O)CC)C6=NN=C(C)N26

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for Biotin BromoCatch™ Ligand

分析证书/产品说明书
化学品安全技术说明书

参考文献 for Biotin BromoCatch™ Ligand

参考文献是支持产品生物活性的出版物。

Rodriguez-Rios et al (2025) BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. biorxiv

If you know of a relevant reference for Biotin BromoCatch™ Ligand, please let us know.

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关键词: Biotin BromoCatch™ Ligand, Biotin BromoCatch™ Ligand supplier, BromoCatch, Ligand, biotin, MR169, self-labeling, tag, covalent, probe, platform, selective, labeling, affinity-based, detection, pull-down, Platform, Self-labeling, Tags, 8939, Tocris Bioscience

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Biotin BromoCatch™ Ligand 实验方案

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