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Polybrene

Cat. No. 7711 1 次引用 1 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Viral transduction enhancer
别名： Hexadimethrine bromide
化学名： 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide
纯度： ≥95%
说明书
引用文献 (1)
评论 (1)
实验方案 (1)
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Polybrene

Polybrene is a cationic polymer that improves lentiviral and adenoviral transduction efficiency in mammalian cells in vitro. It also enhances DNA transfection in many types of cells. It has a neutralizing effect on negatively charged DNA and viral particles which facilitates their attachment and entry into host cells. Polybrene is suitable for use with CRISPR/Cas9 protocols. It is commonly used in combination with Protamine sulfate (Cat. No. 8822) to enhance the delivery of adeno-associated virus (AAV) and lentiviral vectors.

Polybrene is a lyophilized solid and can be prepared as a stock solution by adding 1 mL of sterile ultra-pure water directly to the vial (final stock concentration 10 mg/mL).

For further information view the product protocol.

技术数据 for Polybrene

储存 Store at +4°C
纯度 ≥95%
CAS Number 28728-55-4
Smiles C[N+](C)(CCC[N+](C)(CCCCCCC)C)C.[Br-].[Br-]

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Polybrene

溶解性 Soluble to 100 mg/ml in water

产品说明书 for Polybrene

分析证书/产品说明书
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化学品安全技术说明书

参考文献 for Polybrene

参考文献是支持产品生物活性的出版物。

Davis et al (2002) Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys.Chem. 97 159 PMID: 12050007

Abe et al (1998) Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 5 708 PMID: 9797877

Shayakhmetov et al (2000) Dependence of adenovirus infectivity on length of the fiber shaft domain. J.Virol. 74 10274 PMID: 11044071

Bolis et al (2021) Dynamic prostate cancer transcriptome analysis delineates the trajectory to disease progression. Nat.Commun. 12 7033 PMID: 34857732

Zhao et al (2014) Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene. PLoS One 21 e92908 PMID: 24658746

Navarro-Guerrero et al (2021) Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Sci.Rep. 11 4245 PMID: 33608581

Strack (2022) Breaking entry-and species barriers: LentiBOOST ® Plus polybrene enhances transduction efficacy of dendritic cells and monocytes by adenovirus 5. Viruses 14 92 PMID: 35062296

Kumar et al (2021) Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications. STAR Protoc 2 100874 PMID: 34746857

Joung et al (2022) CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat.Commun 13 1606 PMID: 35338135

Elegheert et al (2018) Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Nat.Protoc. 13 2991 PMID: 30455477

If you know of a relevant reference for Polybrene, please let us know.

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关键词: Polybrene, Polybrene supplier, transduction, transfection, retrovirus, enhancers, gene, delivery, enhances, viral, Hexadimethrine, bromide, Viral, Transduction, Enhancers, Biochemicals, and, Molecular, Biology, 7711, Tocris Bioscience

1 篇 Polybrene 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Polybrene 的部分引用包括：

Yoshitasu et al (2023) Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis. iScience 26 106623 PMID: 37216099

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Polybrene 的评论

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Polybrene.
By Chi Ngo on 09/06/2025
分析类型： In Vitro
种属： Human
细胞系/组织： HepG2

I used Polybrene to enhance the transduction efficiency of retrovirus expressing genes of interest.

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Polybrene 实验方案

以下实验方案包含使用 Polybrene（目录编号 7711）的更多信息。

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