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Submit ReviewJanelia Fluor® 525, BromoCatch™ Ligand 最新
说明： Fluorogenic Janelia Fluor® 525 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
化学名： 4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)benzoate
纯度： ≥90% (HPLC)
生物活性
Janelia Fluor® 525, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 525, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 525 dye, delivering bright signal with minimal background fluorescence. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 525, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.
Please refer to this protocol for further information on how to use this product.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
This product is part of an early adopter program.
BromoCatch™ is a trademark of Bio-Techne Corp.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee
Optical Data for Janelia Fluor® 525, BromoCatch™ Ligand
| λabs | 534 nm |
|---|---|
| λem | 557 nm |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据
| 分子量 | 1146.23 |
| 公式 | C59H59F4N9O9S |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| InChI Key | CIYUOPFNGGEXOY-RWZZRXGDSA-N |
| Smiles | CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCNC(C4=CC=C(C(C5=C6C(OC7=CC(N8CC(F)(F)C8)=CC=C57)=C/C(C=C6)=[N+]9CC(F)(F)C/9)=C4)C([O-])=O)=O)=O)CC)C%10=NN=C(C)N2%10 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书
参考文献
参考文献是支持产品生物活性的出版物。
Rodriguez-Rios et al (2026) BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. Nat. Commun. PMID: 42128888
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关键词: Janelia Fluor® 525 BromoCatch™ Ligand, Janelia Fluor® 525 BromoCatch™ Ligand supplier, Janelia, Fluor525-bromocatch-ligand, JF525, fluorescent, fluorogenic, cell, permeable, real-time, microscopy, live, imaging, BromoCatch-tag, super-resolution, SRM, BromoCatch, Platform, Self-labeling, Tags, 8997, Tocris Bioscience
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