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Submit ReviewChromaLIVE™ Non-Toxic Dye is a ready-to-use fluorescent probe for long term high content phenotypic screening of live-cells. Optimised for live cell painting and morphological profiling in 2D monolayers, 3D spheroids or organoids, the dye provides multi organelle read out for unbiased classification of cell states such as proliferation, stress responses, autophagy, apoptosis or other phenotypic transitions. Excitation and emission maxima (λ) are 488 nM and 561 nM, respectively. ChromaLIVE™ Non-Toxic Dye is provided as 10 μL of 1000x concentrate in DMSO and is compatible with high-throughput and high-content screening workflows, including automated 96-, 384-, and 1536-well plate formats.
Please refer to this protocol for further information on how to use this product.
ChromaLIVE™ is a trademark of Saguaro Biosciences.
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ChromaLIVE™ Non-Toxic Dye Application. High-content imaging application of HeLa cells treated with staurosporine for 20 hours, then stained with ChromaLIVE™ Non-Toxic Dye and Hoechst 33342. Image acquired on the Yokogawa CQ1.
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ChromaLIVE™ Non-Toxic Dye Application. High-content imaging of live MCF-7 cells stained with ChromaLIVE™ Non-Toxic Dye, imaged at 561–600 nm and 488–700 nm. Demonstrates live-cell compatibility and bright cytoplasmic signal.
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ChromaLIVE™ Non-Toxic Dye Application. High-content imaging of HEK293 spheroids stained with ChromaLIVE™ Non-Toxic Dye and imaged using the Yokogawa CQ3000. Six-tile panel displays treatment conditions left to right: DMSO, rapamycin (50 µM), and brefeldin A (10 µM); timepoints top to bottom: 24 h and 48 h.
Sold under license from Saguaro Biosciences
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
| 溶解性 | Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x) |
参考文献是支持产品生物活性的出版物。
Sivagurunathan et al (2025) Alternate dyes for image-based profiling assays. SLAS Discov. PMID: 40885286
Cottet (2023) Live cell painting: new nontoxic dye to probe cell physiology in high content screening. SLAS Discov. S2472 S2472 PMID: 39492484
If you know of a relevant reference for ChromaLIVE™ Non-Toxic Dye, please let us know.
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