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Submit ReviewChromaLIVE™ Non-Toxic Dye is a ready-to-use fluorescent probe for long term high content phenotypic screening of live cells. It allows live cell phenotypic imaging with a single dye enabling multi-organelle phenotypic readout to accurately differentiate cellular phenotypes stress and cell death pathways. Excitation and emission maxima (λ) are 488 nM and 561 nM, respectively. ChromaLIVE™ Non-Toxic Dye is provided as 10 μL of 1000x concentrate in DMSO.
Please refer to this protocol for further information on how to use this product.
ChromaLIVE™ is a registered trademark of Saguaro Biosciences.
ChromaLIVE™ Non-Toxic Dye Application. High-content imaging application of HeLa cells treated with staurosporine for 20 hours, then stained with ChromaLIVE™ Non-Toxic Dye and Hoechst 33342. Image acquired on the Yokogawa CQ1.
ChromaLIVE™ Non-Toxic Dye Application. High-content imaging of live MCF-7 cells stained with ChromaLIVE™ Non-Toxic Dye, imaged at 561–600 nm and 488–700 nm. Demonstrates live-cell compatibility and bright cytoplasmic signal.
ChromaLIVE™ Non-Toxic Dye Application High-content imaging of HEK293 spheroids stained with ChromaLIVE™ Non-Toxic Dye and imaged using the Yokogawa CQ3000. Six-tile panel displays treatment conditions left to right: DMSO, rapamycin (50 µM), and brefeldin A (10 µM); timepoints top to bottom: 24 h and 48 h.
Sold under license from Saguaro Biosciences
λabs | 488 nm |
---|---|
λem | 561 nm |
Cell Permeable | Yes |
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer储存 | Store at -20°C |
纯度 | ≥90% (HPLC) |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性 | Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x) |
参考文献是支持产品生物活性的出版物。
Sivagurunathan et al (2025) Alternate dyes for image-based profiling assays. SLAS Discov. PMID: 40885286
If you know of a relevant reference for ChromaLIVE™ Non-Toxic Dye, please let us know.
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: