ChromaLIVE™ Non-Toxic Dye

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说明: Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells
纯度: ≥90% (HPLC)
说明书
引用文献
评论
实验方案 (1)

生物活性 for ChromaLIVE™ Non-Toxic Dye

ChromaLIVE™ Non-Toxic Dye is a ready-to-use fluorescent probe for long term high content phenotypic screening of live-cells. Optimised for live cell painting and morphological profiling in 2D monolayers, 3D spheroids or organoids, the dye provides multi organelle read out for unbiased classification of cell states such as proliferation, stress responses, autophagy, apoptosis or other phenotypic transitions. Excitation and emission maxima (λ) are 488 nM and 561 nM, respectively. ChromaLIVE™ Non-Toxic Dye is provided as 10 μL of 1000x concentrate in DMSO and is compatible with high-throughput and high-content screening workflows, including automated 96-, 384-, and 1536-well plate formats.

Please refer to this protocol for further information on how to use this product.

ChromaLIVE™ is a trademark of Saguaro Biosciences.

Scientific Data

Live-cell Imaging Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells View Larger

ChromaLIVE™ Non-Toxic Dye Application. High-content imaging application of HeLa cells treated with staurosporine for 20 hours, then stained with ChromaLIVE™ Non-Toxic Dye and Hoechst 33342. Image acquired on the Yokogawa CQ1.

Live-cell Imaging Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells View Larger

ChromaLIVE™ Non-Toxic Dye Application. High-content imaging of live MCF-7 cells stained with ChromaLIVE™ Non-Toxic Dye, imaged at 561–600 nm and 488–700 nm. Demonstrates live-cell compatibility and bright cytoplasmic signal.

Live-cell Imaging Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells View Larger

ChromaLIVE™ Non-Toxic Dye Application. High-content imaging of HEK293 spheroids stained with ChromaLIVE™ Non-Toxic Dye and imaged using the Yokogawa CQ3000. Six-tile panel displays treatment conditions left to right: DMSO, rapamycin (50 µM), and brefeldin A (10 µM); timepoints top to bottom: 24 h and 48 h.

许可信息

Sold under license from Saguaro Biosciences

Optical Data for ChromaLIVE™ Non-Toxic Dye

ChromaLIVE™ Non-Toxic Dye Dye Spectra
λabs 488 nm
λem 561 nm
Cell Permeable Yes

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for ChromaLIVE™ Non-Toxic Dye

储存 Store at -20°C
纯度 ≥90% (HPLC)

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for ChromaLIVE™ Non-Toxic Dye

溶解性 Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x)

产品说明书 for ChromaLIVE™ Non-Toxic Dye

参考文献 for ChromaLIVE™ Non-Toxic Dye

参考文献是支持产品生物活性的出版物。

Sivagurunathan et al (2025) Alternate dyes for image-based profiling assays. SLAS Discov. PMID: 40885286

Cottet (2023) Live cell painting: new nontoxic dye to probe cell physiology in high content screening. SLAS Discov. S2472 S2472 PMID: 39492484


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ChromaLIVE™ Non-Toxic Dye 实验方案

以下实验方案包含使用 ChromaLIVE™ Non-Toxic Dye(目录编号 8934)的更多信息。