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Submit ReviewKey information: Rhobo6 ECM Dye is a cell-impermeable fluorescent probe for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds reversibly to diols commonly found in ECM glycans resulting in a significant increase in fluorescence and a red shift in emission spectra.
Used for: labeling the ECM in live tissues.
Application: fluorescent microscopy including 2P and STED microscopy. Enables wash-free visualization of ECM in vitro and in vivo and is suitable for long-term imaging studies.
Properties and Photophysical Data: Rhobo6 provides broad substrate profile, superior tissue penetration, and negligible photobleaching. Excitation and emission maxima (λ) are 535 nm and 561 nm, respectively; quantum yield = 0.75; extinction coefficient = 97,500 M-1cm-1.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer| 分子量 | 756.26 |
| 公式 | C35H28B2N2O9.CF3CO2H |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| InChI Key | RPHZIIQRPDDKIQ-OWCLOVKRSA-N |
| Smiles | FC(F)(C(O)=O)F.OB(O)C(C=CC=C1)=C1CNC(C=C2)=CC(O3)=C2C(C(C=C(C=C4)C(O)=O)=C4C([O-])=O)=C(C=C/5)C3=CC5=[NH+]/CC6=CC=CC=C6B(O)O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 7.56 | 10 |
以下数据基于产品分子量 756.26。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.22 mL | 66.11 mL | 132.23 mL |
| 0.5 mM | 2.64 mL | 13.22 mL | 26.45 mL |
| 1 mM | 1.32 mL | 6.61 mL | 13.22 mL |
| 5 mM | 0.26 mL | 1.32 mL | 2.64 mL |
参考文献是支持产品生物活性的出版物。
Fiore et al (2025) Live imaging of the extracellular matrix with a glycan-binding fluorophore. Nat.Methods 22 1070 PMID: 39915692
If you know of a relevant reference for Rhobo6 ECM Dye, please let us know.
关键词: Rhobo6 ECM Dye, Rhobo6 ECM Dye supplier, Rhobo6, ECM, dyes, extracellular, matrix, imaging, fluorophores, live, cell, cell-impermeable, labeling, probes, fluorescent, fluorescence, wash-free, in, vivo, tissues, 2P, STED, microscopy, Other, Fluorescent, Probes, Live-cell, Imaging, 8876, Tocris Bioscience
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