Rhobo6 ECM Dye

Pricing Availability   Qty
说明: Fluorogenic probe for labeling the extracellular matrix (ECM) in live tissues
化学名: (E)-2-(6-((2-Boronobenzyl)amino)-3-((2-boronobenzyl)iminio)-3H-xanthen-9-yl)-4-carboxybenzoate trifluoroacetate
纯度: ≥90% (HPLC)
说明书
引用文献
评论

生物活性 for Rhobo6 ECM Dye

Key information: Rhobo6 ECM Dye is a cell-impermeable fluorescent probe for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds reversibly to diols commonly found in ECM glycans resulting in a significant increase in fluorescence and a red shift in emission spectra.

Used for: labeling the ECM in live tissues.

Application: fluorescent microscopy including 2P and STED microscopy. Enables wash-free visualization of ECM in vitro and in vivo and is suitable for long-term imaging studies.

Properties and Photophysical Data: Rhobo6 provides broad substrate profile, superior tissue penetration, and negligible photobleaching. Excitation and emission maxima (λ) are 535 nm and 561 nm, respectively; quantum yield = 0.75; extinction coefficient = 97,500 M-1cm-1.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Rhobo6 ECM Dye

Rhobo6 ECM Dye Dye Spectra
λabs 522 nm
λem 547 nm
Extinction Coefficient (ε) 66,400 M-1cm-1
Quantum Yield (φ) 0.61
Cell Permeable No

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for Rhobo6 ECM Dye

分子量 756.26
公式 C35H28B2N2O9.CF3CO2H
储存 Store at -20°C
纯度 ≥90% (HPLC)
InChI Key RPHZIIQRPDDKIQ-OWCLOVKRSA-N
Smiles FC(F)(C(O)=O)F.OB(O)C(C=CC=C1)=C1CNC(C=C2)=CC(O3)=C2C(C(C=C(C=C4)C(O)=O)=C4C([O-])=O)=C(C=C/5)C3=CC5=[NH+]/CC6=CC=CC=C6B(O)O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Rhobo6 ECM Dye

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 7.56 10

制备储备液 for Rhobo6 ECM Dye

以下数据基于产品分子量 756.26。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 13.22 mL 66.11 mL 132.23 mL
0.5 mM 2.64 mL 13.22 mL 26.45 mL
1 mM 1.32 mL 6.61 mL 13.22 mL
5 mM 0.26 mL 1.32 mL 2.64 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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参考文献 for Rhobo6 ECM Dye

参考文献是支持产品生物活性的出版物。

Fiore et al (2025) Live imaging of the extracellular matrix with a glycan-binding fluorophore. Nat.Methods 22 1070 PMID: 39915692


If you know of a relevant reference for Rhobo6 ECM Dye, please let us know.

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