Pepstatin A Janelia Fluor® 526

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Cat.No. 7314 - Pepstatin A Janelia Fluor 526 | C67H91F6N9O12.CF3CO2H | CAS No. 2410614-07-0
说明: Fluorogenic green-emitting lysosome tracker and stain
别名: Pepstatin A JF526
化学名: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((6S,9S,12S,13S,17S,20S,21S)-13,21-dihydroxy-12,20-diisobutyl-6,9-diisopropyl-2,17-dimethyl-4,7,10,15,18,23-hexaoxo-5,8,11,16,19,24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate
纯度: ≥90% (HPLC)
说明书
引用文献
评论 (1)

生物活性 for Pepstatin A Janelia Fluor® 526

Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome tracker and stain. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Pepstatin A Janelia Fluor® 526

Pepstatin A Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Green
λabs 530 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Application confocal microscopy, STED, 3D-SIM, lattice light-sheet microscopy
Alternative For LysotrackerTM- Lysotracker is a registered trademark of Thermo Fisher

技术数据 for Pepstatin A Janelia Fluor® 526

分子量 1442.53
公式 C67H91F6N9O12.CF3CO2H
储存 Store at -20°C
纯度 ≥90% (HPLC)
CAS Number 2410614-07-0
InChI Key JONSFJPZCPEZFP-WKVYEHBGSA-N
Smiles OC(C(F)(F)F)=O.CC(C[C@@H]([C@H](CC(NCCCCCCNC(C1=CC(C2=C3C=C(/C(C=C3OC4=C2C=C(C(N5CC(F)(C5)F)=C4)F)=[N+]6CC(F)(C\6)F)F)=C(C([O-])=O)C=C1)=O)=O)O)NC([C@@H](NC(C[C@@H]([C@@H](NC([C@H](C(C)C)NC([C@H](C(C)C)NC(CC(C)C)=O)=O)=O)CC(C)C)O)=O)C)=O)C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Pepstatin A Janelia Fluor® 526

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 14.43 10

制备储备液 for Pepstatin A Janelia Fluor® 526

以下数据基于产品分子量 1442.53。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 6.93 mL 34.66 mL 69.32 mL
0.5 mM 1.39 mL 6.93 mL 13.86 mL
1 mM 0.69 mL 3.47 mL 6.93 mL
5 mM 0.14 mL 0.69 mL 1.39 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Dilution Calculator

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产品说明书 for Pepstatin A Janelia Fluor® 526

参考文献 for Pepstatin A Janelia Fluor® 526

参考文献是支持产品生物活性的出版物。

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787


If you know of a relevant reference for Pepstatin A Janelia Fluor® 526, please let us know.

关键词: Pepstatin A Janelia Fluor 526, Pepstatin A Janelia Fluor 526 supplier, Fluorogenic, green, emitting, lysosomes, lysosomal, probes, stains, dyes, tracker, staining, Pepstatin, A, JF526, Fluorescent, Lysosome, Probes, 7314, Tocris Bioscience

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Specific dye.
By Karthik Krishnamurthy on 08/03/2021
分析类型: In Vitro
种属: Rat
细胞系/组织: astrocytes

labeling of lysosomes in astrocytes under basal conditions

review image