Pepstatin A Janelia Fluor® 526

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Cat.No. 7314 - Pepstatin A Janelia Fluor 526 | C67H91F6N9O12.CF3CO2H | CAS No. 2410614-07-0
说明: Fluorogenic green-emitting lysosome tracker and stain
别名: Pepstatin A JF526
化学名: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((6S,9S,12S,13S,17S,20S,21S)-13,21-dihydroxy-12,20-diisobutyl-6,9-diisopropyl-2,17-dimethyl-4,7,10,15,18,23-hexaoxo-5,8,11,16,19,24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate
纯度: ≥90% (HPLC)
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生物活性 for Pepstatin A Janelia Fluor® 526

Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome tracker and stain. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.


Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Pepstatin A Janelia Fluor® 526

Pepstatin A Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Green
λabs 530 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Application confocal microscopy, STED, 3D-SIM, lattice light-sheet microscopy
Alternative For LysotrackerTM- Lysotracker is a registered trademark of Thermo Fisher

技术数据 for Pepstatin A Janelia Fluor® 526

分子量 1442.53
公式 C67H91F6N9O12.CF3CO2H
储存 Store at -20°C
纯度 ≥90% (HPLC)
CAS Number 2410614-07-0
Smiles OC(C(F)(F)F)=O.CC(C[C@@H]([C@H](CC(NCCCCCCNC(C1=CC(C2=C3C=C(/C(C=C3OC4=C2C=C(C(N5CC(F)(C5)F)=C4)F)=[N+]6CC(F)(C\6)F)F)=C(C([O-])=O)C=C1)=O)=O)O)NC([C@@H](NC(C[C@@H]([C@@H](NC([C@H](C(C)C)NC([C@H](C(C)C)NC(CC(C)C)=O)=O)=O)CC(C)C)O)=O)C)=O)C


Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Pepstatin A Janelia Fluor® 526

溶剂 最高浓度 mg/mL 最高浓度 mM
DMSO 14.43 10

制备储备液 for Pepstatin A Janelia Fluor® 526

以下数据基于产品分子量 1442.53。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 6.93 mL 34.66 mL 69.32 mL
0.5 mM 1.39 mL 6.93 mL 13.86 mL
1 mM 0.69 mL 3.47 mL 6.93 mL
5 mM 0.14 mL 0.69 mL 1.39 mL

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产品说明书 for Pepstatin A Janelia Fluor® 526

参考文献 for Pepstatin A Janelia Fluor® 526


Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Pepstatin A Janelia Fluor® 526, please let us know.

关键词: Pepstatin A Janelia Fluor 526, Pepstatin A Janelia Fluor 526 supplier, Fluorogenic, green, emitting, lysosomes, lysosomal, probes, stains, dyes, tracker, staining, Pepstatin, A, JF526, Fluorescent, Lysosome, Probes, 7314, Tocris Bioscience

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Specific dye.
By Karthik Krishnamurthy on 08/03/2021
分析类型: In Vitro
种属: Rat
细胞系/组织: astrocytes

labeling of lysosomes in astrocytes under basal conditions

review image