Hygromycin B

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Cat.No. 4137 - Hygromycin B | C20H37N3O13 | CAS No. 31282-04-9
说明: Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
化学名: O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
纯度: ≥90% (HPLC)
评论 (1)

生物活性 for Hygromycin B

Hygromycin B is an aminoglycoside antibiotic. Active against both prokaryotic and eukaryotic cells. Inhibits protein synthesis by binding to the 30S subunit and inhibiting the ribosomal translocation step of elongation.

技术数据 for Hygromycin B

分子量 527.52
公式 C20H37N3O13
储存 Store at +4°C
纯度 ≥90% (HPLC)
CAS Number 31282-04-9
PubChem ID 90488914
Smiles O[C@H]([C@@H](NC)C[C@@H](N)[C@@H]4O)C4O[C@H]1[C@]2([H])[C@](OC3(O[C@]([H])([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]3O)O2)([H])[C@@H](O)[C@@H](CO)O1


Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Hygromycin B

溶剂 最高浓度 mg/mL 最高浓度 mM
water 50 95

制备储备液 for Hygromycin B

以下数据基于产品分子量 527.52。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.95 mM 2 mL 9.98 mL 19.95 mL
4.75 mM 0.4 mL 2 mL 3.99 mL
9.5 mM 0.2 mL 1 mL 2 mL
47.5 mM 0.04 mL 0.2 mL 0.4 mL

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产品说明书 for Hygromycin B


参考文献 for Hygromycin B


Blochlinger and Diggelmann (1984) Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eukaryotic cells. Mol.Cell.Biol. 4 2929 PMID: 6098829

Brodersen et al (2000) The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell 103 1143 PMID: 11163189

Ganoza and Kiel (2001) A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob.Agents Chemother. 45 2813 PMID: 11557474

If you know of a relevant reference for Hygromycin B, please let us know.


关键词: Hygromycin B, Hygromycin B supplier, hygromycinB, aminoglycoside, antibiotics, inhibits, protein, synthesis, ribosomal, translocation, ribosome, 30S, eukaryotic, prokaryotic, cells, Antibiotics, 4137, Tocris Bioscience

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Hygromycin B 的评论

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Hygromycin used as a selection marker in cells engineered with Hygromycin resistant gene.
By Anonymous on 11/15/2018
分析类型: In Vitro
种属: Human

Hygromycin was used as a selection marker for few eukaryotic cells which were engineered with Hygromycin resistant gene. Product works beautifully to inhibit translation and killing non resistant strains giving it desirable specificity.

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