Y-27632 dihydrochloride

Cat. No. 1254

Y-27632 dihydrochloride C14H21N3O.2HCl [129830-38-2]

Price and Availability

For Y-27632 dihydrochloride pricing & availability please select your country from the drop down menu:
By clicking submit you agree to accept a cookie from Tocris Bioscience. For details, please read our privacy and cookie policy.

Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride

Biological Activity

Selective p160ROCK inhibitor (Ki values are 0.14, 26, 25 and > 250 μM for p160ROCK, PKC, PKA and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Smooth muscle relaxant and orally active in vivo. Increases survival rate of human embryonic stem (hES) cells undergoing cryopreservation. Also optimizes naïve human pluripotent stem cell growth and viability following naïve cell derivation from primed ESCs and iPSCs using naïve human stem cell medium (NHSM).

Technical Data

Soluble to 100 mM in water
>98 %
Desiccate at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Safety Data Sheet

Certificate of Analysis: View current batch
Select another batch:
Safety Data Sheet: View Safety Data Sheet


Uehata et al (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase. Nature 389 990. PMID: 9353125.

Narumiya et al (2000) Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 325 273. PMID: 11036610.

Ichikawa et al (2011) Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling. Cryo Letters. 32 516. PMID: 22227712.

Gafni et al (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature 504 282. PMID: 24172903.

If you know of a relevant citation for this product please let us know.

Keywords: Y-27632 dihydrochloride, supplier, Selective, p160ROCK, inhibitor, Rho-Kinase, Rho-Associated, Coiled-Coil, Kinases, ROCK, ROK, stem, cells

Quick Order

Find multiple products by catalog number

divider line

Kinases Product Listing

Kinases Product Listing

Highlights over 400 products for kinase research. Request a copy or view PDF today.

divider line

Kinase Inhibitor Toolbox

80 Kinase inhibitors
pre-dissolved in DMSO

Kinase Inhibitor Toolbox

Includes many of the latest kinase inhibitors to become commercially available. Covers a wide range of kinase targets including EGFR, MAPK and CDK.

divider line

Cardiovascular Research Product Guide

Cardiovascular Research Guide

Highlights over 250 products for cardiovascular research. Request a copy or view PDF today.

divider line

VEGF Pathway & Anti-Angiogenic Therapy

Written by N. Ferrara & Y. Crawford

Angiogenesis Poster

A summary of antiangiogenic therapies targeting the VEGF pathway and the mechanisms of therapy resistance.

divider line

New Product Guide


Highlights 130 new products added in the first half of 2015. View PDF today.

divider line

New Products in this Area

NG 25

TGF-β-activated kinase (TAK1) inhibitor

MI 14

Potent and selective PI 4-kinase IIIβ inhibitor

CTA 056

ITK inhibitor

AZD 1480

Potent and selective JAK2 inhibitor; antiangiogenic

TC-S 7001

Potent and highly selective ROCK inhibitor; orally active

AS 2444697

Potent and selective IRAK4 inhibitor

BS 181 dihydrochloride

Selective cdk7 inhibitor


Potent and selective PI 3-kinase p110α inhibitor

AZ 191

Potent DYRK1B inhibitor

ETP 45658

Potent PI 3-kinase inhibitor; also inhibits DNA-PK and mTOR

PFK 15

PFKFB3 inhibitor

PD 0332991 isethionate

Potent, selective Cdk4/6 inhibitor; brain penetrant

XL 413 hydrochloride

Potent and selective Cdc7 inhibitor


PKR inhibitor

Rp-8-pCPT-cGMPS sodium

PKG inhibitor

TC-S 7004

Potent and selective DYRK1A/B inhibitor

TAK 960 hydrochloride

Potent and selective PLK1 inhibitor

Sign-up for new product e-alerts
divider line

Bio-Techne Events

One Day Symposium

Bio-Techne Symposium 2015

Stem Cells & Organoids as Models of Tissue Differentiation & Disease

January 12, 2016

London, UK