ATPases are a group of enzymes that catalyze the hydrolysis of a phosphate bond in adenosine triphosphate (ATP) to form adenosine diphosphate (ADP). They harness the energy released from the breakdown of the phosphate bond and utilize it to perform other cellular reactions. ATPases are essential enzymes in all known forms of life and have fundamental roles in energy conservation, active transport and pH homeostasis.

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There are three classes of ATPases, which differ in structure and the type of ion that they transport; F-ATPases (e.g. mitochondrial H+-ATPase), P-ATPases (e.g. Ca2+-ATPase, Na+,K+-ATPase) and V-ATPases (e.g. lysosomal H+-ATPase).

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