Cat. No. 1531

Icilin C16H13N3O4 [36945-98-9]

Price and Availability

For Icilin pricing & availability please select your country from the drop down menu:
By clicking submit you agree to accept a cookie from Tocris Bioscience. For details, please read our privacy and cookie policy.

Alternative Name: AG 3-5

Chemical Name: 3,4-Dihydro-3-(2-hydroxyphenyl)-6-(3-nitrophenyl)-(1H)-pyrimidin-2-one

Biological Activity

Cooling agent that activates the novel cold receptors TRPM8 (CMR1) and TRPA1 (ANKTM1/TRPN1), members of the TRP ion channel family. Induces currents in CMR1-expressing HEK 293 cells (EC50 = 0.36 μM) more potently than menthol or low temperatures. Produces "wet shakes" in vivo.

Technical Data

Soluble to 100 mM in DMSO
>98 %
Store at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Safety Data Sheet

Certificate of Analysis: View current batch
Select another batch:
Safety Data Sheet: View Safety Data Sheet


Wei et al (1983) AG-3-5: a chemical producing sensations of cold. J.Pharm.Pharmacol. 35 110. PMID: 6131976.

McKemy et al (2002) Identification of a cold receptor reveals a general role for TRP channels in thermosensation. Nature 416 52. PMID: 11882888.

Story et al (2003) ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell 112 819. PMID: 12654248.

Behrendt et al (2004) Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay. Br.J.Pharmacol. 141 737. PMID: 14757700.

If you know of a relevant citation for this product please let us know.

View Related Products by Target

View Related Products by Product Action

Keywords: Icilin, supplier, activators, novel, cold, receptor, Cooling, agent, TRP, Channels, Transient, Receptor, Potential, AG35, TRPA, TRPM, TRPA1 Activators

Quick Order

Find multiple products by catalog number

divider line

Pain Research Product Guide

Pain Research Product Guide

Highlights over 280 products for Pain research. Request copy or view PDF today.

divider line

Peripheral Sensitization

Written by Grant D. Nicol and Michael R. Vasko

Pain Life Science Poster

A summary of the excitatory and inhibitory signaling pathways involved in modulation of peripheral sensitization. Request copy or view PDF today.

divider line

New Products in this Area

AMG 21629

Potent and selective TRPV1 antagonist

CIM 0216

Selective TRPM3 agonist

MRS 1477

TRPV1 positive allosteric modulator


TRPML channel activator

Sign-up for new product e-alerts
divider line

Bio-Techne Events

One Day Symposium

Bio-Techne Symposium 2015

Stem Cells & Organoids as Models of Tissue Differentiation & Disease

January 12, 2016

London, UK