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The Tocris Dilution Calculator

Calculate the dilution required to prepare a stock solution

The Tocris dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C₁, C₂ & V₂ to calculate V₁.

Calculate now!

Concentration (start)

x

Volume (start)

=

Concentration (final)

x

Volume (final)

femtomolar picomolar nanomolar micromolar millimolar molar  

nanoliter microliter milliliter liter  

femtomolar picomolar nanomolar micromolar millimolar molar  

microliter milliliter liter

C₁

V₁

C₂

V₂

The dilution calculator equation

The Tocris dilution calculator is based on the following equation:

Concentration_(start) x Volume_(start) = Concentration_(final) x Volume_(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

An example of a dilution calculation using the Tocris dilution calculator

What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?

Using the equation C₁V₁ = C₂V₂, where C₁=10 mM, C₂=50 μM, V₂=10 ml and V₁ is the unknown:

1. Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
2. Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
3. Enter 20 into the Volume (final) box and select the correct unit (milliliter)
4. Press calculate
5. The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

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