Tocris Bioscience - High Performance Life Science Reagents The latest New Products, News and Events from Tocris Bioscience https://www.tocris.com https://resources.tocris.com/images/logos/tocris-biotechne-logo-160.png Tocris Bioscience | High Performance Life Science Reagents https://www.tocris.com Tocris Bioscience is a leading supplier of innovative, high performance life science research reagents 160 51 CT-4 - Potent and selective HDAC8 degrader (PROTAC®) https://www.tocris.com/products/ct-4_8024?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/ct-4_8024
Product: CT-4 (Cat No. 8024)
Category: Histone Deacetylase (HDAC) Degraders
Biological Activity: CT-4 is a potent and selective histone deacetylase 8 (HDAC8) degrader (PROTAC®) (DC50 = 1.8 nM after 24 h, Dmax 97%). Comprises a HDAC8-binding moiety joined by a linker to pomalidomide as the ligand for cereblon (CRBN) protein. Displays 20-fold selectivity over HDAC6 (DC50 = 38 nM after 24 h, Dmax 69%) and it does not degrade other HDACs. Displays significant effect on metastasis of MDA-MB-231 cells, and induces degradation and cell death in T-cell leukemia Jurkat cells. Outperformed HDAC8 inhibitors in oncology models. HDAC8 mediates signalling via scaffolding functions in HDAC8/p53/fusion protein complexes, also interacts with STAT3, cAMP and DEC1 via scaffolding functions. PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license ]]> Histone Deacetylase (HDAC) Degraders New Products 2026-06-02T15:30:00Z SPiDER-beta-Gal Cellular Senescence Dye - Fluorogenic β-galactosidase substrate suitable for live/fixed cells and tissues imaging. https://www.tocris.com/products/spider-beta-gal-cellular-senescence-dye_9034?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/spider-beta-gal-cellular-senescence-dye_9034
Product: SPiDER-βGal Cellular Senescence Dye (Cat No. 9034)
Category: Live-cell Imaging
Biological Activity: Key information: SPiDER-βGal Cellular Senescence Dye is a fluorogenic β-galactosidase substrate suitable for labeling live cells in culture and living tissues. It exhibits activation of fluorescence upon reaction with the enzyme, remains inside cells by anchoring itself to intracellular proteins and provides single-cell resolution. Used for: labeling and imaging lacZ reporters in living cells, tissues and model organisms. Also used to investigate senescence. Application: Confocal microscopy, flow cytometry, fluorescence microscopy. SPiDER-βGal Cellular Senescence Dye has high sensitivity for the detection of peritoneal ovarian cancer metastases in animal models and displays high constrast of tumor margins. SPiDER-βGal Cellular Senescence Dye was used for investigating senescence of pancreatic cells and imaging adhesion G-protein-coupled receptors expressed in endothelial cells. Properties and Photophysical Data: Excitation and emission maxima (λ) measured in 200 mM sodium phosphate buffer are 500-540 nm and 530-570 nm, respectively; activation ratio >650-fold. Please refer to this protocol for further information on how to use this product. ]]> Live-cell Imaging New Products 2026-06-02T15:30:00Z Dibutyryl-cAMP, sodium salt - Dibutyryl-cAMP, sodium salt synthesized to Ancillary Material Grade https://www.tocris.com/products/dibutyryl-camp-sodium-salt_tb1141-rmu?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/dibutyryl-camp-sodium-salt_tb1141-rmu
Product: Dibutyryl-cAMP, sodium salt (Cat No. TB1141-RMU)
Category: Ancillary Material Grade Small Molecules
Biological Activity: Dibutyryl-cAMP sodium salt is a cell-permeable analog of cAMP that activates cAMP-dependent protein kinases and is a phosphodiesterase inhibitor. Combined with other reagents, dibutyryl-cAMP promotes differentiation of neural stem/progenitor cells (NSPCs) and other cell lines (see our protocol below) and increases survival rates of NSPCs and differentiation into neurons in vivo. Promotes neurite outgrowth in cell cultures. Dibutyryl-cAMP has anti-inflammatory activity and is used to promote wound healing. Choline acetyltransferase and vesicular acetylcholine transporter mRNA are increased in cells treated with dibutyryl-cAMP. For more information about how Dibutyryl-cAMP, sodium salt may be used, see our protocol: Generating Midbrain Dopaminergic Neurons from hPSCs This product is Bio-Techne's ancillary Material Grade version of Dibutyryl-cAMP sodium salt (Cat. No. 1141). Suitable for use in research and further manufacturing of cell therapies. Find out more about our Ancillary Material Grade product range. ]]> Ancillary Material Grade Small Molecules New Products 2026-05-26T15:30:00Z RiboDyEE™ - Fluorescent dye for quantification of RNA in LNP encapsulation efficiency assays (1 Pack/5x120μL) https://www.tocris.com/products/ribodyee_9000?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/ribodyee_9000
Product: RiboDyEE™ (Cat No. 9000)
Category: Lipid Nanoparticles
Biological Activity: Key information: RiboDyEE™ RNA Dye is an ultra-sensitive green, fluorescent dye specifically developed in a user-friendly format for RNA-LNP encapsulation efficiency (EE) assays. Rigorously validated against the leading competitor, it delivers equivalent performance, with enhanced convenience and fewer handling steps. Application: RiboDyEE is used for the precise quantification of RNA encapsulated within lipid nanoparticles (LNPs) and can detect low RNA concentrations ranging from 1 to 200 ng. Format: RiboDyEE provides a stable, more usable and cost-effective solution compared to the leading competitor product. Supplied in convenient 120 µL aliquots (5 aliquots per pack), each RiboDyEE aliquot is sufficient for accurately measuring up to 21 RNA samples in total, across 3 x 96-well plates (for each plate: 7 different RNA samples, in triplicate, + 1 blank and calibration curves), using a protocol specifically optimized for determining encapsulation efficiency. Additionally, each aliquot can be used to generate standard calibration curves under two conditions, with and without detergent, enhancing quantification accuracy while minimizing degradation caused by repeated freeze-thaw cycles. Properties and Photophysical Data: The dye exhibits excitation and emission maxima (λ) at 485 nm and 525 nm, respectively, with over 1000-fold fluorescence enhancement and a high quantum yield of 0.65 upon RNA binding. Encapsulation Efficiency Assay: For instructions and expert tips on optimizing encapsulation efficiency using RiboDyEE, please consult the provided protocol and application note. This optimized assay accounts for detergent effects, advises preparing standards from the specific RNA species being encapsulated instead of generic RNA, and delivers accurate measurements of encapsulation efficiency along with the percentage of RNA recovery. Request our data analysis spreadsheet to help with your encapsulation efficiency data analysis. RiboDyEE is a trademark of Bio-Techne Corp. ]]> Lipid Nanoparticles New Products 2026-05-26T15:30:00Z Compound E - Compound E synthesized to Ancillary Material Grade https://www.tocris.com/products/compound-e_tb6476-rmu?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/compound-e_tb6476-rmu
Product: Compound E (Cat No. TB6476-RMU)
Category: Ancillary Material Grade Small Molecules
Biological Activity: Compound E is a γ-secretase and notch pathway inhibitor. Compound E enhances growth inhibition, differentiation and migration of neuroblastoma cells when used in a combination with DAPT (Cat. No. 2634) and 13-cis RA. In combination with hLIF, CHIR 99021 (Cat. No. 4423) and SB 431542 (Cat. No. 1614), Compound E accelerates the induction of a homogeneous, self-renewing primitive neuroepithelium population from hESCs. Compound E is active in vitro and in vivo. Used in protocol to generate pancreatic beta cells from hPSCs (see below). For more information about how Compound E may be used, see our protocol: Generation of β cells from hPSCs This product is Bio-Techne's ancillary Material Grade version of Compound E (Cat. No. 6476). Suitable for use in research and further manufacturing of cell therapies. Find out more about our Ancillary Material Grade product range. ]]> Ancillary Material Grade Small Molecules New Products 2026-05-26T15:30:00Z 1-Azakenpaullone - Potent and selective GSK3 inhibitor. Used in chemical cocktail for induction and maintainance of TotiSCs. https://www.tocris.com/products/1-azakenpaullone_3673?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/1-azakenpaullone_3673
Product: 1-Azakenpaullone (Cat No. 3673)
Category: Glycogen Synthase Kinase 3
Biological Activity: 1-Azakenpaullone is a potent and selective inhibitor of glycogen synthase kinase 3 (GSK-3), with IC50 = 18 nM. It displays selectivity index (SI) of 111 and 233 over CDK1 and CDK5, respectively. 1-Azakenpaullone stimulates growth and replication of β-cells in vitro (rat islets) and alleviates glucolipotoxicity in INS-1 β-cells. It displays mitogenic effects on rat β-cells comparable with those of incretin hormone GIP. In combination with TTNPB (Cat. No. 0761) and WS 6 (Cat. No. 7791), 1-Azakenpaullone can be part of a chemical cocktail used for induction and long-term maintainance in vitro of totipotent stem cells (TotiSCs) from mouse pluripotent stem cells (PSCs). ]]> Glycogen Synthase Kinase 3 New Products 2026-05-05T15:30:00Z b-Lipotropin 61-91 - Endogenous opioid peptide; component of the LIP cocktail for preconditioning human β-cells https://www.tocris.com/products/b-lipotropin-61-91_8984?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/b-lipotropin-61-91_8984
Product: β-Lipotropin 61-91 (Cat No. 8984)
Category: μ Opioid Receptors
Biological Activity: β-Lipotropin 61-91 (or β-Endorphin) is an endogenous opioid peptide with potent antinociceptive activity, physiologically released in response to painful stimuli. β-Lipotropin 61-91 can be used to study the expression of μ-opioid receptor in organ cultures and to investigate cardiovascular regulation. In combination with 10 μM Prostaglandin E2 (Cat. No. 2296) and 1 μg/mL IGF-1, β-Lipotropin 61-91 (0.01 μM) constitutes the LIP cocktail, a small molecule mixture used to precondition human β-cells and primary islets, significantly enhancing function and survival when transplanted subcutaneously to female mice. ]]> μ Opioid Receptors New Products 2026-05-05T15:30:00Z VVD-214 - Potent WRN covalent allosteric inhibitor; orally bioavailable https://www.tocris.com/products/vvd-214_9027?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/vvd-214_9027
Product: VVD-214 (Cat No. 9027)
Category: DNA, RNA and Protein Synthesis
Biological Activity: VVD-214 is a potent werner syndrome helicase (WRN) covalent allosteric inhibitor (IC50 = 0.13 μM and 2.4 μM with and without ATP, respectively). The selective binding of VVD-214 to cysteine C727 of WRN triggers double-stranded DNA breaks, nuclear swellings, cell death and tumor regression in microsatellite instability-high (MSI-H) colorectal cancer models. Highly stable in hepatocytes and whole blood, VVD-214 is orally bioavailable. ]]> DNA, RNA and Protein Synthesis New Products 2026-05-05T15:30:00Z Semaglutide - Highly potent, long-acting GLP-1 receptor agonist https://www.tocris.com/products/semaglutide_8946?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/semaglutide_8946
Product: Semaglutide (Cat No. 8946)
Category: Glucagon-Like Peptide 1 Receptors
Biological Activity: Semaglutide is a glucon-like peptide 1 analog and a potent and long-acting GLP-1R agonist. Upon activating GLP-1R, Semaglutide promotes insulin secretion, inhibits gastric emptying and appetite, causing lasting and reversible weight loss in normal and obese rats. Semaglutide enhances autophagy by increasing the expression of LC3II, Atg7, Beclin-1 and P62; inhibits oxidative stress and apoptosis. Semaglutide has neuroprotective properties in a stroke animal model, where neurogenesis and growth factor cell signaling is normalized. Semaglutide can be used for the study of neurodegenerative diseases and liver diseases such as type 2 diabetes, obesity, Parkinson's disease, metabolic associated fatty liver disease (MASLD), and cancer. ]]> Glucagon-Like Peptide 1 Receptors New Products 2026-05-05T15:30:00Z SALAD1 - Ultrabright fluorogenic dye for imaging RNA in living cells; activated by binding Mango aptamers https://www.tocris.com/products/salad1_8974?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/salad1_8974
Product: SALAD1 (Cat No. 8974)
Category: Aptamer-based RNA Imaging
Biological Activity: Key information: SALAD1 is a specific and high-affinity aptamer-ligand for aptamer Mango II (KDapp = 0.69 nM). Green fluorogenic dye displaying 3.5-fold brighter fluorescence than other Mango aptamer dyes such as TO1-biotin. Binds 7.5-fold more tightly to Mango II compared to TO dye. SALAD1 exhibits a binding preference to its target RNA (Mango II) over the other three Mango aptamers (7-, 20- and 16-fold tighter than Mango I, III and IV). The improved brightness and tighter binding of SALAD1 to Mango aptamers allow detection and tracking of RNA with greater sensitivity and spatial resolution, facilitating studies of RNA localization, dynamics, and expression in live-cell and single-molecule imaging applications. Used for: imaging RNA in live and fixed cells, even at low concentration. Application: confocal cellular imaging. Properties and Photophysical Data: excitation and emission maxima (λ) measured in Mango selection buffer are 511 nm and 540 nm, respectively; quantum yield QY = 0.722; extinction coefficient ε(complex) = 47,284 M-1cm-1; fluorescence enhancement FE = 1,500; fluorescence efficiency E = 2,170 nM-1; brightness = 34,139 M-1cm-1. SALAD1 is cell-permeable. ]]> Aptamer-based RNA Imaging New Products 2026-04-07T15:30:00Z LTGO-33 - Potent and selective state-independent inhibitor of NaV1.8 sodium channels https://www.tocris.com/products/ltgo-33_8865?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/ltgo-33_8865
Product: LTGO-33 (Cat No. 8865)
Category: Voltage-gated Sodium (NaV) Channels
Biological Activity: LTGO-33 is a potent and selective state-independent inhibitor of NaV1.8 sodium channels (IC50 = 13 nM for the closed state; IC50 = 24 nM for the inactive state). It displays 600-fold selectivity over other sodium channels such as NaV1.1-1.7 and NaV1.9. By binding to NaV1.8 channels, LTGO-33 inhibits action potential initiation and transmission of pain signals in human DRG neurons. Unlike other NaV1.8 inhibitors that preferentially interact with an inactivated state via the pore region, LTGO-33 is state-independent with similar potencies against closed and inactivated channels. ]]> Voltage-gated Sodium (Na<sub>V</sub>) Channels New Products 2026-03-31T15:30:00Z LipoLIVE&trade; Green Non-Toxic Dye - Ready-to-use fluorescent probe for the real-time visualization of lipid droplet dynamics in living cells https://www.tocris.com/products/lipolive-green-non-toxic-dye_9026?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/lipolive-green-non-toxic-dye_9026
Product: LipoLIVE™ Green Non-Toxic Dye (Cat No. 9026)
Category: Live-cell Imaging
Biological Activity: LipoLIVE™ Green Non-Toxic Dye is a fluorescent probe for the real-time visualization of lipid droplet and neutral lipids in live cells. It does not require cell fixation or affect cell health and maintains the physiological integrity of models, enabling free-wash monitoring of lipid synthesis, storage, and mobilization. Designed for high-content screening (HCS) workflows, it offers exceptional signal-to-noise ratios and photostability, making it the ideal partner for metabolic dysfunction-associated steatohepatitis (MASH), neurodegeneration, and oncology studies (demonstrated staining of U2OS and iPSC derived neurons). It is provided as 50 μL of a 1000× concentrate in DMSO. Excitation and emission maxima (λ) are 488 nm and 525-550 nm, respectively. Please refer to this protocol for further information on how to use this product. LipoLIVE™ is a trademark of Saguaro Biosciences. ]]> Live-cell Imaging New Products 2026-03-24T16:30:00Z Biotin-PEG4-Diazirine - Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle https://www.tocris.com/products/biotin-peg4-diazirine_8994?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/biotin-peg4-diazirine_8994
Product: Biotin-PEG4-Diazirine (Cat No. 8994)
Category: Biochemicals and Molecular Biology
Biological Activity: Key information:Biotin-PEG4-Diazirine is a cell-permeable photo-crosslinking probe for elucidating protein interactome of polyarginine cell-penetrating peptides and mapping cell surfaceome through deazaflavin-diazirine energy-transfer labelling (DarT labelling). Upon UV irradiation and in the presence of deazaflavin as an organic photocatalyst, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby proteins. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. Suitable for live-cell applications. Used for: photocatalytic proximity labelling. Application: deazaflavin-diazirine energy-transfer labelling (DarT labelling) followed by affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures. ]]> Biochemicals and Molecular Biology New Products 2026-03-24T16:30:00Z PIPE 3297 dihydrochloride - Potent and selective &#954; opioid receptor agonist; devoid of &#946;-Arrestin-2 recruitment. Also induces differentiation of OPC. https://www.tocris.com/products/pipe-3297_8135?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/pipe-3297_8135
Product: PIPE 3297 dihydrochloride (Cat No. 8135)
Category: κ Opioid Receptors
Biological Activity: PIPE 3297 dihydrochloride is a potent and selective agonist of κ opioid receptor (KOR). It potently activates G-protein signaling (GTPγS EC50 = 1.1 nM, 91% Emax). It displays 40- and 600- fold selectivity over MOR and DOR respectively. PIPE 3297 dihydrochloride induces differentiation of oligodendrocyte progenitor cells (OPC) in vitro and in vivo, leading to significant increase of mature oligodendrocytes (OL) in healthy mice and inducing myelination. It also reduces disease score in mouse experimental autoimmune encephalomyelitis (EAE) model. Exhibits low β-arrestin2 recruitment activity, avoding the sedating effects typically associated to other KOR agonists employed in demyelinating diseases. PIPE 3297 dihydrochloride is brain penetrant and leads to 90% occupancy in CNS in vivo (mouse). PIPE 3297 exhibits cardiotoxity with 72% hERG inhibition (3 μM) and unstability in liver microsomes. ]]> &kappa; Opioid Receptors New Products 2026-03-17T16:30:00Z Janelia Fluor&reg; 552, Haloalkane - Cell-permeable fluorogenic dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate. Also chemigenetic Ca2+ indicator. https://www.tocris.com/products/janelia-fluor-552-haloalkane_8948?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/janelia-fluor-552-haloalkane_8948
Product: Janelia Fluor® 552, Haloalkane (Cat No. 8948)
Category: Self-labeling Tags
Biological Activity: Key Information: Janelia Fluor® 552, Haloalkane is a yellow cell-permeable fluorogenic dye with a chloroalkane handle. Also chemigenetic calcium indicator for imaging Ca2+ mediated responses. Upon self-labeling in WHaloCaMP modular biosensors, fluorescence output is modulated by photoinduced electron transfer (PET) to a protein based quencher. Application: live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). In vivo functional imaging of Ca2+ responses (flies/mice); multiplexed functional imaging of neurons and astrocytes in zebrafish larvae; quantification of Ca2+ concentration using fluorescence lifetime imaging microscopy (FLIM). Properties and Photophysical Data: Stable to fixation. Cell permeable. KD = 87 ± 5. In the presence of EGTA (Ca2+ chelator) excitation and emission maxima (λ) are 561 nm and 559-578 nm, respectively; quantum yield = 0.05; extinction coefficient = 73,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); brigthness = 3.6 mM-1cm-1. In the presence of saturating Ca2+, excitation and emission maxima (λ) are 562 nm and 565-582 nm, respectively; quantum yield = 0.25; extinction coefficient = 78,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); brigthness = 19.5 mM-1cm-1. Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute. ]]> Self-labeling Tags New Products 2026-03-17T16:30:00Z Janelia Fluor&reg; 570, NHS ester - Orange dye (Reactive group: NHS ester). Application: Suitable for live cell imaging https://www.tocris.com/products/janelia-fluor-570-nhs-ester_8983?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/janelia-fluor-570-nhs-ester_8983
Product: Janelia Fluor® 570, NHS ester (Cat No. 8983)
Category: Amine-Reactive Janelia Fluor® Dyes
Biological Activity: Key information: Janelia Fluor® 570, NHS ester is an orange photosensitizer for proximity labeling experiments; supplied with an NHS ester reactive for the labeling of primary amines. Suitable for live cell applications. Upon absorption of light energy, photosensitizers in the triplet excited state interact with molecular oxygen resulting in the formation of singlet oxygen and other ROS that can be leveraged to manipulate or interrogate biological systems. Used for: proximity labeling experiments. Application: photosensitizer-dependent oxidation and capture by amine (POCA) platforms reporting intracellular interactomes for both proteins and lipids such as cholesterol. Also suitable for chromophore-assisted light inactivation (CALI) of proteins in live cells; targeted ablation of cells; DAB photopolymerization for contrast in light and electron microscopy (EM). Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. Haloalkane and SNAP-tag®. Excitation and emission maxima (λ) are 570 nm and 593 nm, respectively; quantum yield φ = 0.63; extinction coefficient ε = 83,600 M-1cm-1; singlet oxygen quantum yield φSO = 0.088; lactone-zwitterion equilibrium constant (KL-Z) = 2.24. Janelia Fluor® 570, NHS ester is net neutral and cell permeable. Protocol: for cell-based POCA: cells are dosed with JF570-NHS (100 nM, 10 min) followed by washout to remove excess reagent (2 x 20 min), addition of PA (10 mM) and irradiation (170,000 Lux, 5 min,15 W yellow LED). After cell lysis, click chemistry and SDS-PAGE are performed. Please see the protocol for further information on protein/antibody labeling and conjugation. We also offer ]]> Amine-Reactive Janelia Fluor<sup>&#174;</sup> Dyes New Products 2026-03-10T16:30:00Z Plitidepsin - Potent protein synthesis inhibitor https://www.tocris.com/products/plitidepsin_7416?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/plitidepsin_7416
Product: Plitidepsin (Cat No. 7416)
Category: DNA, RNA and Protein Synthesis
Biological Activity: Plitidepsin (dehydrodidemnin B) is a potent inhibitor of protein synthesis, blocker of G1 and G2 phases in cell cycle and potent antiviral against SARS-CoV-2 in hACE2-293T, Vero E6 cells and models of pneumocyte-like cells (IC90 values are 0.88 nM, 1.76 nM and 3.14 nM, respectively). Plitidepsin also inhibits eukaryotic translation elongation factor 1 alpha 1 (eEF1A) (KD = 80 nM). In vitro, it inhibits induction of ornithine decarboxylase (ODC), which is essential for cell transformation and proliferation. In vivo, daily administration of Plitidepsin on mice affected by Ehrlich carcinoma duplicated the animal life-span and decreased the total number of tumor cells by 70-90%. Plitidepsin also reduces viral SARS-CoV-2 replication in the lungs (mouse). It is recommended to prepare stock solutions in DMSO. ]]> DNA, RNA and Protein Synthesis New Products 2026-03-10T16:30:00Z ASP 7967 - Ligand for RNA aptamer AC17-4 activating gene expression in aptazime-based riboswitches https://www.tocris.com/products/asp-7967_8803?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/asp-7967_8803
Product: ASP 7967 (Cat No. 8803)
Category: DNA, RNA and Protein Synthesis
Biological Activity: ASP 7967 is a ligand for RNA aptamer AC17-4 that activates gene expression in synthetic aptazime-based riboswitches at as low as 5 μM concentration, without relying on exogenous protein factors. ASP 7967 binds aptamer AC17-4 with high affinity in HEK293 cells (Kd = 12 nM; EC50 = 2.3 μM), activating gene expression (>10-fold) in HEK293 cells. ON/OFF ratio of riboswitches activated by ASP 7967 approaches 300, with a low basal expression level in cultured cells. In vivo, ASP 7967 upregulates the serum hEPO concentration by 7.2-fold over the vehicle control (mice). Cell permeable and low-toxic, ASP 7967 is orally bioavailable. ]]> DNA, RNA and Protein Synthesis New Products 2026-02-24T16:30:00Z Janelia Fluor&reg; 549, BromoCatch&trade; Ligand - Fluorogenic Janelia Fluor&reg; 549 conjugated covalent ligand for BromoCatch&trade;, enabling no-wash live-cell imaging https://www.tocris.com/products/janelia-fluor-549-bromocatch-ligand_8942?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/janelia-fluor-549-bromocatch-ligand_8942
Product: Janelia Fluor® 549, BromoCatch™ Ligand (Cat No. 8942)
Category: BromoCatch™ Platform
Biological Activity: Janelia Fluor® 549, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 549, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 549 dye, delivering bright signal with minimal background fluorescence. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 549, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast. Please refer to this protocol for further information on how to use this product. Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute. This product is part of an early adopter program. BromoCatch™ is a trademark of Bio-Techne Corp. ]]> BromoCatch<sup>&trade;</sup> Platform New Products 2026-02-17T16:30:00Z KSQ 4279 - Potent and selective USP1 allosteric inhibitor https://www.tocris.com/products/ksq-4279_7746?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/ksq-4279_7746
Product: KSQ 4279 (Cat No. 7746)
Category: Deubiquitinating Enzymes
Biological Activity: KSQ 4279 is a potent and selective allosteric inhibitor of the ubiquitin specific protease 1 (USP1) (IC50 = 11 nM; Ki = 1.2 nM), a key regulator of DNA damage repair pathway (distinct from PARP inhibitors). It displays selectivity for USP1 over other USP, UCH and OTU enzymes. KSQ 4279 has strong antiproliferation effects in vitro. It induces accumulation of mono-ubiquitinated substrates in MDA-MB-436 cells. In vivo, KSQ 4279 induces anti-tumor response in the BRCA1 mutant ovarian PDX model (mouse). It also displays synergistic effects with PARP inhibitor Olaparib (Cat. No. 7579) leading to significant and durable anti-tumor response in mice. KSQ 4279 is orally bioavailable. ]]> Deubiquitinating Enzymes New Products 2026-02-17T16:30:00Z Bleomycin sulfate - DNA synthesis inhibitor. Induces pulmonary inflammation and fibrosis https://www.tocris.com/products/bleomycin-sulfate_7147?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/bleomycin-sulfate_7147
Product: Bleomycin sulfate (Cat No. 7147)
Category: DNA, RNA and Protein Synthesis
Biological Activity: Bleomycin sulfate is a glycopeptide antibiotic produced by Streptomyces verticillus. It inhibits DNA synthesis and causes single and double-strand breaks at specific base sequences in intracellular DNA of bacterial and mammalian cells. It also acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis. It is an anticancer agent for squamous cell carcinomas (SCC) with IC50 = 4 nM in UT-SCC-19A cells. In vivo, Bleomycin sulfate induces pulmonary inflammation and fibrosis in lung injury models (mice). Bleomycin sulfate contains two main components: 1) Bleomycin A2 hemisulfate, C55H84N17O21S3.HSO4, molecular weight = 1512.62 Da 2) Bleomycin B2, C55H84N20O21S2, molecular weight = 1425.52 Da. The exact proportions vary from batch to batch and can be found on the relevant certificate of analysis. ]]> DNA, RNA and Protein Synthesis New Products 2026-02-17T16:30:00Z CX-4945 - Potent and selective CK2&#945; and CK2&#945;' inhibitor. Also blocks CK2-dependent HIF-1&#945; transcription, causes cell-cycle arrest, induces apoptosis in cancer cells and induces stem cell reprogramming https://www.tocris.com/products/cx-4945_8993?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/cx-4945_8993
Product: CX-4945 (Cat No. 8993)
Category: Casein Kinase 2
Biological Activity: CX-4945 (Silmitasertib) is a potent and selective inhibitor of protein kinase CK2α and CK2α' (IC50 = 1 nM). It shows greater than 500-fold selectivity for CK2 over a panel of > 200 kinases. CX-4945 can be used, in combination with other small molecules, in fast chemical reprogramming protocols for the induction of human somatic cells and highly efficient generation of pluripotent stem cells (hCiPS) in a minimum of 16 days with robust repeatability and reliability. CX-4945 has antiproliferative effects in vitro as it attenuates PI3K/Akt signalling, causes cell-cycle arrest and selectively induces apoptosis in cancer cells. In models of angiogenesis, CX-4945 inhibits human umbilical vein endothelial cell migration, tube formation, and blocks CK2-dependent hypoxia-induced factor 1 alpha (HIF-1α) transcription in cancer cells. In vivo, CX-4945 displays robust antitumor activity with concomitant reduction of the mechanism-based biomarker phospho-p21 (T145) (murine xenograft model). CX-4945 is orally bioavailable. ]]> Casein Kinase 2 New Products 2026-02-10T16:30:00Z NAIA - Broad spectrum cysteine reactive probe; suitable for live-cell applications https://www.tocris.com/products/naia_8910?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/naia_8910
Product: NAIA (Cat No. 8910)
Category: Biochemicals and Molecular Biology
Biological Activity: NAIA (N-Acryloylindole-alkyne) is a broad-spectrum cysteine reactive probe enabling cysteine profiling and imaging. It covalently binds cysteine residues in proteins. Displays faster reaction kinetics with CYS (k > 102 M-1 s-1) and targets an expanded pool of ligandable cysteines and proteins compared to IA-Alkyne (Cat. No. 7015). Also shows higher stability and selectivity for CYS over SER and LYS than IA-Alkyne (Cat. No. 7015). NAIA is used in chemoproteomics experiments as a click-chemistry handle to attach a fluorophore or biotin to reactive cysteines in proteins for visualization by in-gel fluorescence or identification by mass spectrometry, respectively. NAIA can also be used to image oxidized cysteines in cells under oxidative stress by confocal fluorescence microscopy. Cell-permeable and non-cytotoxic, NAIA is suitable for live-cell experiments. ]]> Biochemicals and Molecular Biology New Products 2026-02-10T16:30:00Z Okadaic acid ammonium salt - Protein phosphatase 1 and 2A inhibitor https://www.tocris.com/products/okadaic-acid-ammonium-salt_9012?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/okadaic-acid-ammonium-salt_9012
Product: Okadaic acid ammonium salt (Cat No. 9012)
Category: Protein Ser/Thr Phosphatases
Biological Activity: Okadaic acid ammonium salt is a potent inhibitor of protein phosphatase 1 (IC50 = 3 nM) and protein phosphatase 2A (IC50 = 0.2-1 nM). Displays > 100,000,000-fold selectivity over PP2B and PP2C. Tumor promoter. Shown to activate atypical protein kinase C in adipocytes. ]]> Protein Ser/Thr Phosphatases New Products 2026-02-03T16:30:00Z VVD 844 - Potent covalent inhibitor of the RAS domain of the PI3K/p110&#945; interaction https://www.tocris.com/products/vvd-844_8952?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/vvd-844_8952
Product: VVD 844 (Cat No. 8952)
Category: Ras GTPases
Biological Activity: VVD 844 is a potent inhibitor of the PI3K/p110α interaction (IC50 = 4 nM) and the phosphorylated-Ser473-AKT (pAKT) pathway (IC50 = 11 nM). It covalently binds cysteine 242 in the RAS domain of PI3K/p110α and blocks RAS activation of PI3Kα activity in vitro. In vivo, VVD 844 slows growth of RAS mutant tumors and human epidermal growth factor receptor 2 (HER2)-overexpressing tumors, without causing hyperglycemia. Exhibits synergistic effects with Sotorasib (Cat. No. 7713) and Adagrasib (Cat. No. 7488). VVD 844 is orally bioavailable. ]]> Ras GTPases New Products 2026-02-03T16:30:00Z FerroOrange - Sensitive and cell-permeable fluorogenic probe for the detection of labile Fe(II) in live cells; suitable for high-throughput assays https://www.tocris.com/products/ferroorange_8846?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/ferroorange_8846
Product: FerroOrange (Cat No. 8846)
Category: Fluorescent Ion Indicators
Biological Activity: Key information: FerroOrange (RhoNox-4) is a highly sensitive and robust fluorogenic probe for the detection of intracellular labile Fe(II). FerroOrange displays weak absorption and low fluorescence in the visible region, but becomes strongly fluorescent upon reaction with Fe(II). Used for: detection of intracellular labile Fe(II). Application: high-throughput assays for high content imaging of subcellular labile Fe(II) in live cells; fluorescent microscopy; studies on iron homeostasis and ferroptosis. Properties and Photophysical Data: FerroOrange has excellent cell-membrane permeability and low cell toxicity. FerroOrange has no chelating ability. For the fluorescent form, excitation and emission maxima (λ) measured in 50 mM HEPES buffer (pH 7.4, 0.2% DMSO) are 545 nm and 575 nm, respectively; extinction coefficient ε = 73,500 M-1cm-1; quantum yield φ = 0.19. Please refer to this protocol for further information on how to use this product. ]]> Fluorescent Ion Indicators New Products 2026-02-03T16:30:00Z Janelia Fluor&reg; 525 BromoCatch&trade; Ligand - Fluorogenic Janelia Fluor&reg; 525 conjugated covalent ligand for BromoCatch&trade;, enabling no-wash live-cell imaging https://www.tocris.com/products/janelia-fluor-525-bromocatch-ligand_8997?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/janelia-fluor-525-bromocatch-ligand_8997
Product: Janelia Fluor® 525, BromoCatch™ Ligand (Cat No. 8997)
Category: BromoCatch™ Platform
Biological Activity: Janelia Fluor® 525, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 525, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 525 dye, delivering bright signal with minimal background fluorescence. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 525, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast. Please refer to this protocol for further information on how to use this product. Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute. This product is part of an ]]> BromoCatch<sup>&trade;</sup> Platform New Products 2026-01-20T16:30:00Z Janelia Fluor&reg; 585, BromoCatch&trade; Ligand - Fluorogenic Janelia Fluor&reg; 585 conjugated covalent ligand for BromoCatch&trade;, enabling no-wash live-cell imaging https://www.tocris.com/products/janelia-fluor-585-bromocatch-ligand_8998?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/janelia-fluor-585-bromocatch-ligand_8998
Product: Janelia Fluor® 585, BromoCatch™ Ligand (Cat No. 8998)
Category: BromoCatch™ Platform
Biological Activity: Janelia Fluor® 585, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 585, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 585 dye, delivering bright signal with minimal background fluorescence. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 585, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast. Please refer to this protocol for further information on how to use this product. Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute. This product is part of an early adopter program. BromoCatch™ is a trademark of Bio-Techne Corp. ]]> BromoCatch<sup>&trade;</sup> Platform New Products 2026-01-13T16:30:00Z Liperfluo - Fluorescent lipid peroxide probe for live cell imaging https://www.tocris.com/products/liperfluo_8868?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/liperfluo_8868
Product: Liperfluo (Cat No. 8868)
Category: Fluorescent Lipid Probes and Cell Membrane Stains
Biological Activity: Key information: Liperfluo is a fluorescent probe for the selective detection and imaging of lipid peroxides in living cells. Upon reaction with lipid peroxides, Liperfluo is oxidized and becomes fluorescent. Used for: imaging lipid peroxides in living cells. Liperfluo can be used to monitor lipid peroxidation in ferroptosis research. Application: fluorescent microscopy and flow cytometry. Properties and Photophysical Data: Liperfluo has a high solubility in organic solvents. It displays minimal photo damage and auto-fluorescence and shows high selectivity to lipid peroxidases over ROS and other radical species. Excitation and emission maxima (λ) measured in ethanol are 524 nm and 535 nm, respectively; quantum yield (oxidized form) = 0.85. Please refer to this protocol for further information on how to use this product. ]]> Fluorescent Lipid Probes and Cell Membrane Stains New Products 2026-01-13T16:30:00Z HG-9-91-01 - Potent pan-salt inducible kinases (SIK1, SIK2 and SIK3) inhibitor. Also inhibits Lat-B-induced EP differentiation via nuclear YAP1 accumulation. https://www.tocris.com/products/hg-9-91-01_8985?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/hg-9-91-01_8985
Product: HG-9-91-01 (Cat No. 8985)
Category: Other Kinases
Biological Activity: HG-9-91-01 (SIK1 inhibitor) is a potent pan-salt inducible kinases (pan-SIKs) inhibitor (IC50 values are 0.92, 6.6 and 9.6 nM for SIK1, SIK2 and SIK3 respectively). HG-9-91-01 inhibits latrunculin B (Lat-B)-induced endocrine progenitors (EP) differentiation via nuclear YAP1 accumulation in vitro. HG-9-91-01 increases LPS-stimulated IL-10 production and suppresses pro-inflammatory cytokine secretion in vitro. HG-9-91-01 increases β-cell proliferation in vitro and in vivo (mouse, zebrafish). ]]> Other Kinases New Products 2026-01-13T16:30:00Z RO-275 - Potent and selective HCN1 inhibitor https://www.tocris.com/products/ro-275_8977?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/ro-275_8977
Product: RO-275 (Cat No. 8977)
Category: HCN Channels
Biological Activity: RO-275 is a potent and selective HCN1 (hyperpolarization-activated cyclic-nucleotide-gated channel 1) inhibitor (IC50 = 46 nM). It shows <100-fold selectivity over HCN2, HCN3 and HCN4. In vivo, RO-275 improves performance in a translationally relevant touchscreen task of working memory (TUNL) in rats. RO-275 is orally bioavailable and brain-penetrant. ]]> HCN Channels New Products 2025-12-16T16:30:00Z NVP-TNKS656 - Tankyrase (AKA PARP5) inhibitor; used to generate neurons from epiblast stem cells https://www.tocris.com/products/nvp-tnks656_8913?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/nvp-tnks656_8913
Product: NVP-TNKS656 (Cat No. 8913)
Category: Tankyrase
Biological Activity: NVP-TNKS656 is a potent inhibitor of tankyrase enzymes (pIC50 values are 7.8 and 8.2 for TNKS1 and TNKS2 respectively). It displays >5000 selectivity over PARP1 and PARP2. In vivo, it disrupts Wnt pathway activity in the MMTV-Wnt1 mouse xenograft model. NVP-TNKS656 is used to generate neurons from epiblast stem cells. NVP-TNKS656 is orally bioavailable. ]]> Tankyrase New Products 2025-12-16T16:30:00Z NucleoLIVE&trade; Blue Non-Toxic Dye - Ready-to-use fluorescent probe for long term high content nuclear imaging in live cells https://www.tocris.com/products/nucleolive-blue-non-toxic-dye_9011?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/nucleolive-blue-non-toxic-dye_9011
Product: NucleoLIVE™ Blue Non-Toxic Dye (Cat No. 9011)
Category: Live-cell Imaging
Biological Activity: NucleoLIVE™ Blue Non-Toxic Dye is a ready-to-use fluorescent probe for long term high content nuclear imaging in live cells. NucleoLIVE™ Blue, is the same as the original NucleoLIVE™ Non-Toxic Dye (Red), but with different spectral properties, allowing compatibility with ChromaLIVE™ Non-Toxic Dye (Cat. No.8934). Ideal for long term 2D and 3D cell organoids high-content imaging and real-time cell viability assays. NucleoLIVE™ Blue Non-Toxic Dye facilitates cell counting to accurately quantify cell toxicity, viability, and morphology by fluorescence on the blue channel, and can be used to generate growth curves to quantify drug effects on cell proliferation in real time and over multiple days. Allow wash-free imaging. NucleoLIVE™ Blue Non-Toxic Dye is provided as 50 µL of 1000x concentrate in DMSO. Please refer to this protocol for further information on how to use this product. NucleoLIVE™ is a trademark of Saguaro Biosciences. ]]> Live-cell Imaging New Products 2025-12-09T16:30:00Z ChromaLIVE&trade; Deep Red Non-Toxic Dye - Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells, can be multiplexed with NucleoLIVE https://www.tocris.com/products/chromalive-deep-red-non-toxic-dye_9009?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/chromalive-deep-red-non-toxic-dye_9009
Product: ChromaLIVE™ Deep Red Non-Toxic Dye (Cat No. 9009)
Category: Live-cell Imaging
Biological Activity: ChromaLIVE™ Deep Red Non-Toxic Dye is a ready-to-use fluorescent probe for long term high content phenotypic screening of live cells. Optimised for live cell painting and morphological profiling in 2D monolayers, 3D spheroids or organoids, the dye provides multi organelle read out for unbiased classification of cell states such as proliferation, stress responses, autophagy, apoptosis or other phenotypic transitions. ChromaLIVE™ Deep Red Non-Toxic Dye can be used with NucleoLIVE™ Non-Toxic Dye (Cat. No. 8935) to get even more insight from live cell painting assays. Please refer to this protocol for further information on how to use this product. ChromaLIVE™ and NucleoLIVE™ are trademarks of Saguaro Biosciences. ]]> Live-cell Imaging New Products 2025-12-09T16:30:00Z ER-LIVE&trade; Endoplasmic Reticulum Dye - Ready-to-use fluorescent probe for long term imaging of ER in live cells, can be multiplexed with ChromaLIVE Deep Red for phenotypic screening https://www.tocris.com/products/er-live-endoplasmic-reticulum-dye_9010?amp;utm_campaign=RSS&utm_source=RSS&utm_medium=RSS https://www.tocris.com/products/er-live-endoplasmic-reticulum-dye_9010
Product: ER-LIVE™ Endoplasmic Reticulum Dye (Cat No. 9010)
Category: Live-cell Imaging
Biological Activity: ER-LIVE™ Endoplasmic Reticulum Dye is a ready-to-use fluorescent probe for long term imaging of ER in live cells. ER-LIVE™ Endoplasmic Reticulum Dye can be multiplexed with ChromaLIVE Deep Red Non-Toxic Dye (Cat. No. 9009) for phenotypic screening. Excitation and emission maxima (λ) are 488 nM and 530 nM, respectively. Please refer to these protocols: ER-LIVE™ Protocol and ChromaLIVE™ Deep red and ER-LIVE™ Protocol for further information on how to use this product. ER-LIVE™ is a trademark of Saguaro Biosciences. ]]> Live-cell Imaging New Products 2025-12-09T16:30:00Z