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This is intended as a guide only; for full experimental details please read the reference provided.
Reconstitute the aptamer at 30-100 µM in sterile deionized H2O and ensure the aptamer has fully dissolved. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials protected from light at -20°C or below and used within 1 month. Wherever possible solutions should be made up and used on the same day. Repeated freeze thaw cycles should be avoided.
Prior to use, dilute the aptamer at an assay dependent concentration in PBS buffer containing 5 mM MgCl2. Heat the solution at 95˚C for 5 min, and incubate for 15 min at room temperature.
Please refer to Gomes de Castro et al, for further information.
Gomes de Castro et al (2017) Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy. 12 e0173050.PMID: 28235049