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Submit ReviewEp23, 5'-DY647
Discontinued Product
Ep23, 5'-DY647 (Cat. No. 6102) has been withdrawn from sale for commercial reasons.
Description: High affinity fluorescently tagged aptamer for EpCam
Biological Activity for Ep23, 5'-DY647
Ep23, 5'-DY647 is a high affinity fluorescently tagged aptamer for EpCam (Kd = 39.42 nM in HT29 cells). Efficiently internalized upon binding to EpCAM. Exhibits rapid penetration of tumorspheres in vitro, as well as superior penetration, accumulation and retention in tumor xenografts in vivo than an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Licensing Information
Sold under license from the patent holder, Deakin University, Australia.
Technical Data for Ep23, 5'-DY647
| Aptamer type (DNA/RNA) | RNA |
| Number of Bases | 19 |
| Sequence Modifications | 2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag |
| M. Wt | 6782.21 |
| Formula | C218H259F13N66O132P20 |
| Storage | Store at -20°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility for Ep23, 5'-DY647
Please refer to these solubility guidelines for Ep23, 5'-DY647 (Cat. No. 6102).
Product Datasheets for Ep23, 5'-DY647
References for Ep23, 5'-DY647
References are publications that support the biological activity of the product.
Shigdar et al (2011) RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule. Cancer Sci. 102 991 PMID: 21281402
Shigdar et al (2013) The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One. 8 e57613 PMID: 23460885
Xiang et al (2015) Superior performance of aptamer in tumor penetration over antibody: implication of aptamer-based theranostics in solid tumors. Theranostics. 5 1083 PMID: 26199647
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Keywords: Ep23, 5'-DY647, Ep23, 5'-DY647 supplier, Ep23-5-DY647, Aptamers, EpCAM, epithelial, cell, adhesion, molecules, fluorescent, high, affinity, Cell, Adhesion, Molecules, Cancer, Stem, Cells, Fluorescent, Surface, Markers, 6102, Tocris Bioscience
Citations for Ep23, 5'-DY647
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals