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High affinity fluorescently tagged aptamer for EpCam (Kd = 39.42 nM in HT29 cells). Efficiently internalized upon binding to EpCAM. Exhibits rapid penetration of tumorspheres in vitro, as well as superior penetration, accumulation and retention in tumor xenografts in vivo than an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Sold under license from the patent holder, Deakin University, Australia.
Application of Ep23, 5'-DY647 in Immunocytochemistry
Aptamer applied at 100 nM concentration to HT29 cells. Magnification 40x.
Image kindly provided by Birte Aggeler, Bio-Techne.
|Aptamer type (DNA/RNA)||RNA|
|Number of Bases||19|
|Sequence Modifications||2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag|
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Please refer to these solubility guidelines for Ep23, 5'-DY647 (Cat. No. 6102).
References are publications that support the biological activity of the product.
Shigdar et al (2011) RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule. Cancer Sci. 102 991 PMID: 21281402
Shigdar et al (2013) The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One. 8 e57613 PMID: 23460885
Xiang et al (2015) Superior performance of aptamer in tumor penetration over antibody: implication of aptamer-based theranostics in solid tumors. Theranostics. 5 1083 PMID: 26199647
If you know of a relevant reference for Ep23, 5'-DY647, please let us know.
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Protocols for Ep23, 5'-DY647
The following protocol features additional information for the use of Ep23, 5'-DY647 (Cat. No. 6102).
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