High affinity fluorescently tagged aptamer for EpCam (Kd = 39.42 nM in HT29 cells). Efficiently internalized upon binding to EpCAM. Exhibits rapid penetration of tumorspheres in vitro, as well as superior penetration, accumulation and retention in tumor xenografts in vivo than an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the accompanying refolding protocol.
Sold under license from the patent holder, Deakin University
|Storage||Store at -20°C|
|Aptamer type (DNA/RNA)||RNA|
|Number of Bases||19|
|Sequence Modifications||2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Please refer to these solubility guidelines for Ep23, 5'-DY647 (Cat.No. 6102).
Preparing Stock Solutions
The following data is based on the product molecular weight 6782.21. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||0.15 mL||0.74 mL||1.47 mL|
|5 mM||0.03 mL||0.15 mL||0.29 mL|
|10 mM||0.01 mL||0.07 mL||0.15 mL|
|50 mM||0 mL||0.01 mL||0.03 mL|
References are publications that support the products' biological activity.
Shigdar et al (2011) RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule. Cancer Sci. 102 991 PMID: 21281402
Shigdar et al (2013) The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One. 8 e57613 PMID: 23460885
Xiang et al (2015) Superior performance of aptamer in tumor penetration over antibody: implication of aptamer-based theranostics in solid tumors. Theranostics. 5 1083 PMID: 26199647
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