High affinity fluorescently tagged aptamer for EpCam (Kd = 39.42 nM in HT29 cells). Efficiently internalized upon binding to EpCAM. Exhibits rapid penetration of tumorspheres in vitro, as well as superior penetration, accumulation and retention in tumor xenografts in vivo than an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the accompanying protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Sold under license from the patent holder, Deakin University, Australia.
Application of Ep23, 5'-DY647 in Immunocytochemistry
Aptamer applied at 100 nM concentration to HT29 cells. Magnification 40x.
Image kindly provided by Birte Aggeler, Bio-Techne.
|Storage||Store at -20°C|
|Aptamer type (DNA/RNA)||RNA|
|Number of Bases||19|
|Sequence Modifications||2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Please refer to these solubility guidelines for Ep23, 5'-DY647 (Cat.No. 6102).
Preparing Stock Solutions
The following data is based on the product molecular weight 6782.21. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||0.15 mL||0.74 mL||1.47 mL|
|5 mM||0.03 mL||0.15 mL||0.29 mL|
|10 mM||0.01 mL||0.07 mL||0.15 mL|
|50 mM||0 mL||0.01 mL||0.03 mL|
References are publications that support the products' biological activity.
Shigdar et al (2011) RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule. Cancer Sci. 102 991 PMID: 21281402
Shigdar et al (2013) The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One. 8 e57613 PMID: 23460885
Xiang et al (2015) Superior performance of aptamer in tumor penetration over antibody: implication of aptamer-based theranostics in solid tumors. Theranostics. 5 1083 PMID: 26199647
If you know of a relevant reference for Ep23, 5'-DY647, please let us know.
View Related Products by Target
Keywords: Ep23, 5'-DY647, supplier, Ep23-5-DY647, Aptamers, EpCAM, epithelial, cell, adhesion, molecules, fluorescent, high, affinity, Fluorescent, Probes, Cell, Adhesion, Molecules, Cancer, Stem, Cells, Aptamers, Aptamers, Tocris Bioscience
Citations for Ep23, 5'-DY647
Citations are publications that use Tocris products.
Currently there are no citations for Ep23, 5'-DY647. Do you know of a great paper that uses Ep23, 5'-DY647 from Tocris? If so please let us know.
Reviews for Ep23, 5'-DY647
There are currently no reviews for this product. Be the first to review Ep23, 5'-DY647 and earn rewards!
Have you used Ep23, 5'-DY647?
Submit a review and receive an Amazon gift card.
$10US/$10CAN/€7/£6 gift card for a review without an image
$25US/$25CAN/€18/£15 gift card for a review with an image
*Offer only valid in the USA / Canada, UK and EuropeSubmit a Review
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.