High affinity fluorescently tagged aptamer for CD133 (extracellular domaine epitope) (Kd values are 52.3 and 145.1 nM in Hep3B and HT29 cells, respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the accompanying protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Sold under license from the patent holder, Deakin University.
|Storage||Store at -20°C|
|Aptamer type (DNA/RNA)||RNA|
|Number of Bases||19|
|Sequence Modifications||2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Please refer to these solubility guidelines for CD133-B19, 5'-DY647 (Cat.No. 6104).
Preparing Stock Solutions
The following data is based on the product molecular weight 6847.32. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||0.15 mL||0.73 mL||1.46 mL|
|5 mM||0.03 mL||0.15 mL||0.29 mL|
|10 mM||0.01 mL||0.07 mL||0.15 mL|
|50 mM||0 mL||0.01 mL||0.03 mL|
References are publications that support the products' biological activity.
Shigdar et al (2013) RNA aptamers targeting cancer stem cell marker CD133. Cancer Lett. 330 84 PMID: 23196060
If you know of a relevant reference for CD133-B19, 5'-DY647, please let us know.
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Keywords: CD133-B19, 5'-DY647, supplier, CD133-B19-5-DY647, Aptamers, CD133, extracellular, domain, epitope, fluorescent, high, affinity, Prominin-1, Fluorescent, Probes, Cancer, Stem, Cells, Aptamers, Aptamers, Tocris Bioscience
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