High affinity fluorescently tagged aptamer for CD133 (extracellular domaine epitope) (Kd values are 52.3 and 145.1 nM in Hep3B and HT29 cells, respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the accompanying protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Sold under license from the patent holder, Deakin University.
|Storage||Store at -20°C|
|Aptamer type (DNA/RNA)||RNA|
|Number of Bases||19|
|Sequence Modifications||2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Please refer to these solubility guidelines for CD133-B19, 5'-DY647 (Cat.No. 6104).
References are publications that support the products' biological activity.
Shigdar et al (2013) RNA aptamers targeting cancer stem cell marker CD133. Cancer Lett. 330 84 PMID: 23196060
If you know of a relevant reference for CD133-B19, 5'-DY647, please let us know.
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Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.