CD133-A15, 5'-DY647
Biological Activity
High affinity fluorescently tagged aptamer for CD133 (AC133 epitope) (Kd values are 33.9 and 83.2 nM in Hep3B and HT29 cells respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Licensing Information
Sold under license from the patent holder, Deakin University.
Application of CD133-A15, 5'-DY647 in Immunocytochemistry
Aptamer applied at 100 nM concentration to HT29 cells. Magnification 40x.
Image kindly provided by Birte Aggeler, Bio-Techne.
Technical Data
| M. Wt | 5549.58 |
| Formula | C182H219F9N58O104P16 |
| Storage | Store at -20°C |
| Aptamer type (DNA/RNA) | RNA |
| Number of Bases | 15 |
| Sequence Modifications | 2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility
Please refer to these solubility guidelines for CD133-A15, 5'-DY647 (Cat. No. 6103).
Product Datasheets
References
References are publications that support the biological activity of the product.
Shigdar et al (2013) RNA aptamers targeting cancer stem cell marker CD133. Cancer Lett. 330 84 PMID: 23196060
If you know of a relevant reference for CD133-A15, 5'-DY647, please let us know.
View Related Products by Target
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Citations for CD133-A15, 5'-DY647
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Protocols for CD133-A15, 5'-DY647
The following protocol features additional information for the use of CD133-A15, 5'-DY647 (Cat. No. 6103).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cancer Research Product Guide
A collection of over 750 products for cancer research, the guide includes research tools for the study of:
- Cancer Metabolism
- Epigenetics in Cancer
- Receptor Signaling
- Cell Cycle and DNA Damage Repair
- Angiogenesis
- Invasion and Metastasis
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.
