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Submit ReviewCD133-A15, 5'-DY647
Description: High affinity fluorescently tagged aptamer for CD133 (AC133 epitope)
Biological Activity
High affinity fluorescently tagged aptamer for CD133 (AC133 epitope) (Kd values are 33.9 and 83.2 nM in Hep3B and HT29 cells respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Licensing Information
Sold under license from the patent holder, Deakin University.
Application of CD133-A15, 5'-DY647 in Immunocytochemistry
Aptamer applied at 100 nM concentration to HT29 cells. Magnification 40x.
Image kindly provided by Birte Aggeler, Bio-Techne.
Technical Data
| Aptamer type (DNA/RNA) | RNA |
| Number of Bases | 15 |
| Sequence Modifications | 2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag |
| M. Wt | 5549.58 |
| Formula | C182H219F9N58O104P16 |
| Storage | Store at -20°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility
Please refer to these solubility guidelines for CD133-A15, 5'-DY647 (Cat. No. 6103).
Product Datasheets
References
References are publications that support the biological activity of the product.
Shigdar et al (2013) RNA aptamers targeting cancer stem cell marker CD133. Cancer Lett. 330 84 PMID: 23196060
If you know of a relevant reference for CD133-A15, 5'-DY647, please let us know.
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Citations for CD133-A15, 5'-DY647
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Protocols for CD133-A15, 5'-DY647
The following protocol features additional information for the use of CD133-A15, 5'-DY647 (Cat. No. 6103).
Literature in this Area
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