CD133-A15, 5'-DY647

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Cat.No. 6103 - CD133-A15, 5'-DY647 | C182H219F9N58O104P16
Description: High affinity fluorescently tagged aptamer for CD133 (AC133 epitope)

Biological Activity

High affinity fluorescently tagged aptamer for CD133 (AC133 epitope) (Kd values are 33.9 and 83.2 nM in Hep3B and HT29 cells respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.

For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the accompanying protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.

Licensing Information

Sold under license from the patent holder, Deakin University.

Application of CD133-A15, 5'-DY647 in Immunocytochemistry

Application of CD133-A15, 5'-DY647 in Immunocytochemistry

Aptamer applied at 100 nM concentration to HT29 cells. Magnification 40x.

Image kindly provided by Birte Aggeler, Bio-Techne.

Technical Data

M. Wt 5549.58
Formula C182H219F9N58O104P16
Storage Store at -20°C
Aptamer type (DNA/RNA) RNA
Number of Bases 15
Sequence Modifications 2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.


Please refer to these solubility guidelines for CD133-A15, 5'-DY647 (Cat.No. 6103).

Preparing Stock Solutions

The following data is based on the product molecular weight 5549.58. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.18 mL 0.9 mL 1.8 mL
5 mM 0.04 mL 0.18 mL 0.36 mL
10 mM 0.02 mL 0.09 mL 0.18 mL
50 mM 0 mL 0.02 mL 0.04 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Shigdar et al (2013) RNA aptamers targeting cancer stem cell marker CD133. Cancer Lett. 330 84 PMID: 23196060

If you know of a relevant reference for CD133-A15, 5'-DY647, please let us know.

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Citations for CD133-A15, 5'-DY647

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