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Discontinued ProductCD133-A15, 5'-DY647 (Cat. No. 6103) has been withdrawn from sale for commercial reasons.
Biological Activity for CD133-A15, 5'-DY647
CD133-A15, 5'-DY647 is a high affinity fluorescently tagged aptamer for CD133 (AC133 epitope) (Kd values are 33.9 and 83.2 nM in Hep3B and HT29 cells respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Sold under license from the patent holder, Deakin University.
Application of CD133-A15, 5'-DY647 in Immunocytochemistry for CD133-A15, 5'-DY647
Aptamer applied at 100 nM concentration to HT29 cells. Magnification 40x.
Image kindly provided by Birte Aggeler, Bio-Techne.
Technical Data for CD133-A15, 5'-DY647
|Aptamer type (DNA/RNA)||RNA|
|Number of Bases||15|
|Sequence Modifications||2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag|
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility for CD133-A15, 5'-DY647
Please refer to these solubility guidelines for CD133-A15, 5'-DY647 (Cat. No. 6103).
Product Datasheets for CD133-A15, 5'-DY647
References for CD133-A15, 5'-DY647
References are publications that support the biological activity of the product.
Shigdar et al (2013) RNA aptamers targeting cancer stem cell marker CD133. Cancer Lett. 330 84 PMID: 23196060
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Citations for CD133-A15, 5'-DY647
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Currently there are no citations for CD133-A15, 5'-DY647.
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
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