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Submit ReviewAnti-human EGFR aptamer, Janelia Fluor® 646 conjugate
Discontinued Product
Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate (Cat. No. 6556) has been withdrawn from sale for commercial reasons.
Description: High affinity anti-human EGFR aptamer conjugated to Janelia Fluor® 646
Biological Activity
Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate is a high affinity anti-human EGFR aptamer conjugated to Janelia Fluor® 646 (Kd = 0.62 nM). Exhibits high affinity and specificity for human epidermal growth factor receptor (EGFR). Displays dense labeling of the stained structures. Suitable for use in super-resolution microscopy techniques.
For optimal binding, aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Technical Data
| Aptamer type (DNA/RNA) | DNA |
| Number of Bases | 50 |
| Sequence Modifications | 3'-inverted deoxythymidine cap, 5'-fluorescent Janelia Fluor® 646 tag |
| M. Wt | 18281.03 |
| Storage | Store at -20°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility
Please refer to these solubility guidelines for Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate (Cat. No. 6556).
Product Datasheets
References
References are publications that support the biological activity of the product.
Kim et al (2014) Efficient isolation and elution of cellular proteins using aptamer-mediated protein precipitation assay. Biochem.Biophys.Res.Commun. 448 114 PMID: 24768638
Gomes de Castro et al (2017) Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy. Send to 12 e0173050 PMID: 28235049
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Protocols for Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate
The following protocol features additional information for the use of Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate (Cat. No. 6556).
Literature in this Area
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Fluorescent Probes and Dyes Research Product Guide
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals