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Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate is a high affinity anti-human EGFR aptamer conjugated to Janelia Fluor® 646 (Kd = 0.62 nM). Exhibits high affinity and specificity for human epidermal growth factor receptor (EGFR). Displays dense labeling of the stained structures. Suitable for use in super-resolution microscopy techniques.
For optimal binding, aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
|Aptamer type (DNA/RNA)||DNA|
|Number of Bases||50|
|Sequence Modifications||3'-inverted deoxythymidine cap, 5'-fluorescent Janelia Fluor® 646 tag|
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Please refer to these solubility guidelines for Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate (Cat. No. 6556).
References are publications that support the biological activity of the product.
Kim et al (2014) Efficient isolation and elution of cellular proteins using aptamer-mediated protein precipitation assay. Biochem.Biophys.Res.Commun. 448 114 PMID: 24768638
Gomes de Castro et al (2017) Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy. Send to 12 e0173050 PMID: 28235049
Keywords: Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate, Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate supplier, Aptamers, epidermal, growth, factor, receptors, EGFRs, Janelia, fluor, 646, JF646, JF_646, conjugates, labels, dyes, fluorescent, super, resolution, microscopy, cell, permeable, EGFR, Fluorescent, Cell, Surface, Markers, 6556, Tocris Bioscience
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