Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate
Biological Activity
High affinity anti-human EGFR aptamer conjugated to Janelia Fluor® 646 (Kd = 0.62 nM). Exhibits high affinity and specificity for human epidermal growth factor receptor (EGFR). Displays dense labeling of the stained structures. Suitable for use in super-resolution microscopy techniques.
For optimal binding, aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Technical Data
| M. Wt | 18281.03 |
| Storage | Store at -20°C |
| Aptamer type (DNA/RNA) | DNA |
| Number of Bases | 50 |
| Sequence Modifications | 3'-inverted deoxythymidine cap, 5'-fluorescent Janelia Fluor® 646 tag |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility
Please refer to these solubility guidelines for Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate (Cat. No. 6556).
Product Datasheets
References
References are publications that support the biological activity of the product.
Kim et al (2014) Efficient isolation and elution of cellular proteins using aptamer-mediated protein precipitation assay. Biochem.Biophys.Res.Commun. 448 114 PMID: 24768638
Gomes de Castro et al (2017) Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy. Send to 12 e0173050 PMID: 28235049
If you know of a relevant reference for Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate, please let us know.
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Protocols for Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate
The following protocol features additional information for the use of Anti-human EGFR aptamer, Janelia Fluor® 646 conjugate (Cat. No. 6556).
Literature in this Area
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