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Psoralen-triethylene glycol azide New
Biological Activity for Psoralen-triethylene glycol azide
Psoralen TEG Azide is used to probe RNA structure and conformation in live cells, using the cross-linking of matched RNAs and deep sequencing or COMRADES method. Psoralen crosslinks the RNA and the azide enables subsequent click reaction with biotin. The COMRADES method has been used to determine the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs. It has also been used to map RNA-RNA interactions along the SARS-CoV-2 genome and subgenomes inside cells. Psoralen TEG Azide is cell permeable. For detailed methods please see references section below.
Technical Data for Psoralen-triethylene glycol azide
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Psoralen-triethylene glycol azide
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Psoralen-triethylene glycol azide
The following data is based on the product molecular weight 458.52. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.18 mL||10.9 mL||21.81 mL|
|5 mM||0.44 mL||2.18 mL||4.36 mL|
|10 mM||0.22 mL||1.09 mL||2.18 mL|
|50 mM||0.04 mL||0.22 mL||0.44 mL|
Product Datasheets for Psoralen-triethylene glycol azide
References for Psoralen-triethylene glycol azide
References are publications that support the biological activity of the product.
Ziv et al (2020) The short- and long-range RNA-RNA interactome of SARS-CoV-2. Mol. Cell 80 1067 PMID: 33259809
Ziv et al (2018) COMRADES determines in vivo RNA structures and interactions. Nat. Methods 15 785 PMID: 30202058
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Citations for Psoralen-triethylene glycol azide
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