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Fluorescent probe for DNA and RNA; amine-reactive. Also shown to stain telomeres. Nuclease resistant and nuclei permeable. Excitation\emission λ ~545\580 nm.
Optical Data for 5-TAMRA SE
|Extinction Coefficient (ε)||84000 M-1cm-1|
|Quantum Yield (φ)||0.1|
|Closest Laser line||532 nm|
|Reactive Group||NHS ester|
|Correction Factor 260||0.32|
|Correction Factor 280||0.19|
|Alternative For||Alexa Fluor(R) 546|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 527.52. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.9 mL||9.48 mL||18.96 mL|
|5 mM||0.38 mL||1.9 mL||3.79 mL|
|10 mM||0.19 mL||0.95 mL||1.9 mL|
|50 mM||0.04 mL||0.19 mL||0.38 mL|
References are publications that support the biological activity of the product.
Hirata et al (2014) Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres. J.Am.Chem.Soc. 136 11546 PMID: 25036716
Kvach et al (2009) Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug. Chem. 20 1673 PMID: 19606815
If you know of a relevant reference for 5-TAMRA SE, please let us know.
Keywords: 5-TAMRA SE, 5-TAMRA SE supplier, 5-TAMRASE, amine-reactive, yellow, fluorescent, labels, Telomerase, Other, Fluorescent, Dyes, 5441, Tocris Bioscience
Citations for 5-TAMRA SE
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Protocols for 5-TAMRA SE
The following protocol features additional information for the use of 5-TAMRA SE (Cat. No. 5441).
Literature in this Area
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