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MitoBrilliant probes have been developed by Tocris to overcome some common limitations encountered with standard mitochondria staining, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells.
Learn more by clicking Background (below), or download the MitoBrilliant Product Guide for more details on the key features of MitoBrilliant™ dyes.
|Cat. No.||Product Name / Activity|
|7700||MitoBrilliant™ 646 New|
|Universal red fluorescent mitochondrial stain for both live and fixed cells|
|7693||MitoBrilliant™ Live 549 New|
|Orange fluorescent mitochondrial stain for live cells, Δψm dependent|
|7417||MitoBrilliant™ Live 646 New|
|Red fluorescent mitochondrial stain for live cells, Δψm dependent|
Our Mitobrilliant range harnesses Janelia Fluor® dye technology, conferring some of the properties of these widely used dyes, into mitochondrial stains. Two types of MitoBrilliant probes are available. The MitoBrilliant Live dyes accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner. Upon loss of the mitochondrial membrane potential, the dyes disperse, and so provide a dynamic assessment of Δψm in live cells. Two live-cell dyes are available: MitoBrilliant™ Live 646 (Cat. No. 7417) (red emission) and MitoBrilliant™ Live 549 (Cat. No. 7693) (yellow/orange emission). MitoBrilliant™ 646 (Cat. No. 7700) is a highly versatile 'universal dye' for both live and fixed cell applications. It is retained in mitochondria following fixation with exceptionally clear staining and is retained in mitochondria following loss of the mitochondrial membrane potential. Additionally, MitoBrilliant™ 646 can be used to stain pre-fixed cells and tissue, making this dye suitable for IHC and ICC.
TABLE 1. Key features of MitoBrilliant™ dyes
|Product Name||Core dye structure||Abs/Em (nm)||Δψm dependent||Live/Fixed cell use||Stain pre-fixed cells?||Image without wash step||Demonstrated Applications|
| MitoBrilliant™ 646 |
Cat. No. 7700
|Janelia Fluor® technology||655/668||No*||Suitable for both live and fixed cell work||Yes||Yes, but replacing media recommended|| Fixed-cell imaging, |
Super-resolution, microscopy – STED,
| MitoBrilliant™ Live 646 |
Cat. No. 7417
|Janelia Fluor® technology||648/662||Yes||Live-cell work only||No||Yes, but replacing media recommended|| Live-cell imaging, |
| MitoBrilliant™ Live 549 |
Cat. No. 7693
|Janelia Fluor® technology||550/568||Yes||Live-cell work only||No||Yes, but replacing media recommended|| Live-cell imaging, |
* In live-cell staining, mitochondrial membrane potential drives initial recruitment of the dye into mitochondria. After staining, localization of the dyes becomes insensitive to the membrane potential changes. In pre-fixed cell staining, MitoBrilliant™ 646 can localize and stay in mitochondria without the membrane potential (see the Product Guide for details).
Figure 1: Images highlighting the superior brightness of MitoBrilliant™ Live 549 compared with the leading competitor mitochondrial stain. HeLa cells were incubated with MitoBrilliant™ Live 549 (100 nM, right) or an equivalent competitor dye (100 nM, left) for 40 minutes. Cells were counterstained with DAPI (Cat. No. 5758). Images obtained using an LSM880 Confocal with a 63X objective. The same settings were used for both images. Scale bar = 10 μm
Figure 2: Images highlighting the improved staining resolution of MitoBrilliant™ 646 compared with the leading competitor mitochondrial stain. HeLa cells were incubated with MitoBrilliant™ 646 (100 nM, right) or an equivalent competitor dye (100 nM, left) for 40 minutes. Cells were then fixed for 10 minutes in 4% PFA. Images obtained using an LSM880 Confocal with a 63X oil objective. Laser power and gain were set to each individual probe. Scale bar = 10 μm.
High content imaging (HCI) is widely used in high-throughput screening and drug discovery. To maximize data capture for HCI, the fluorescent dyes need to be cell-permeable for live-cell applications, bright for desired signal-to-noise ratio in low light levels, photostable for repetitive imaging and exhibit low toxicity in different cell types, as well as minimal interference with normal cell functions. Our MitoBrilliant™ dyes meet all these criteria allowing long-term imaging. MitoBrilliant™ Live dyes can be used for detecting loss of mitochondrial membrane potential, while MitoBrilliant™ 646 can stably label mitochondria during cellular events that might affect the membrane potential, making these dyes excellent tools for functional imaging of mitochondria.
Learn more about our MitoBrilliant dyes in our poster "Novel Live-cell and Fixable Mitochondrial Probes for Cytometry and Imaging".
MitoBrilliant is a trademark of Bio-Techne Corp.
Tocris offers the following scientific literature for MitoBrilliant™ Dyes to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.