DFHBI 1T

Cat. No. 5610

DFHBI 1T C13H9F5N2O2 [1539318-36-9]

Price and Availability

For DFHBI 1T pricing & availability please select your country from the drop down menu:
Submit
By clicking submit you agree to accept a cookie from Tocris Bioscience. For details, please read our privacy and cookie policy.

Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one

Biological Activity

Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

Technical Data

M.Wt:
320.21
Formula:
C13H9F5N2O2
Solubility:
Soluble to 100 mM in DMSO and to 50 mM in ethanol
Purity:
>98 %
Storage:
Store at -20°C
CAS No:
1539318-36-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

References are publications that support the products' biological activity.

Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415. PMID: 26877022.

Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649. PMID: 26000751.

Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198. PMID: 24393009.

If you know of a relevant reference for DFHBI 1T please let us know.

Citations are publications that use Tocris products.

Do you know of a great paper that uses DFHBI 1T from Tocris? If so please let us know.

View Related Products by Target

View Related Products by Product Action

Keywords: DFHBI 1T, supplier, DFHBI1T, fluorescence, fluorophores, RNA, imaging, Spinach2, localization, green, fluorescent, protein, Broccoli, Tocris Bioscience, Fluorescent Probe products

Quick Order

Find multiple products by catalog number

divider line

New Product Guide

Spring/Summer 2017

New Product Guide (Spring/Summer 2017)

Our New Product guide highlights over 130 new products added in the first half of 2017. View PDF today.

divider line

New Products in this Area

PA Janelia Fluor® 646, SE

Photoactivatable cell permeable fluorescent dye; supplied as NHS ester

PA Janelia Fluor® 549, SE

Photoactivatable cell permeable fluorescent dye; supplied as NHS ester

Cyanine 7, SE

Near-infrared fluorescent dye for labeling of amines

Cyanine 3, SE

Orange fluorescent dye for labeling of amines

FFN 200 dihydrochloride

Selective fluorescent VMAT2 substrate

Janelia Fluor® 646, SE

Cell permeable fluorescent dye; supplied as NHS ester

Janelia Fluor® 549, SE

Cell permeable fluorescent dye; supplied as NHS ester

Sign-up for new product e-alerts
divider line

LinkedIn Updates

Follow Tocris on LinkedIn

Follow us on LinkedIn!

Visit our LinkedIn page for the latest Tocris news, events and updates.