Cat. No. 5609

DFHBI C12H10F2N2O2 [1241390-29-3]

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Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one

Biological Activity

Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 447 nm and peak fluorescence emission of 501 nm when bound to Spinach2.

Technical Data

Soluble to 100 mM in DMSO
>98 %
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Safety Data Sheet

Certificate of Analysis: View current batch
Safety Data Sheet: View Safety Data Sheet

Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415. PMID: 26877022.

Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649. PMID: 26000751.

Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198. PMID: 24393009.

Strack et al (2014) Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat.Protoc. 9 146. PMID: 24356773.

Paige et al (2011) RNA mimics of green fluorescent protein. Science 333 642. PMID: 21798953.

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Keywords: DFHBI, supplier, fluorescence, fluorophores, RNA, imaging, Spinach2, localization, green, fluorescent, protein, Broccoli, Tocris Bioscience, Fluorescent Probe products

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