Cat. No. 5342
Chemical Name: 2,3-Dihydro-6,7-diphenyl-2-thioxo-4
Biological ActivityEnhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).
We have discovered that the commercially available SCR7 material supplied by other vendors does not match the chemical structure that it is being sold under. Through detailed chemical analysis carried out by our Analytical Quality Control experts, we believe that the commercially available SCR7 material, including that used in recent publications to enhance CRISPR efficiency, is in fact an analog of SCR7 that we have named 'SCR7 pyrazine'.
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Certificate of Analysis / Product Datasheet / Safety Datasheet
References are publications that support the products' biological activity.
Greco et al (2016) Synthesis and structure determination of SCR7, a DNA ligase inhibitor Teterahedron Lett. 57 3204.
Greco et al (2016) SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV. DNA Repair (Amst) 43 18. PMID: 27235626.
Chu et al (2015) Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat.Biotechnol. 33 543. PMID: 25803306.
Maruyama et al (2015) Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat.Biotechnol. 33 538. PMID: 25798939.
If you know of a relevant reference for SCR7 pyrazine please let us know.
Citations are publications that use Tocris products.
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Keywords: SCR7 pyrazine, supplier, CRISPR, Cas9, HDR, NHEJ, homology, directed, repair, nonhomologous, end, joining, Tocris Bioscience, CRISPR Reagent products