Calcein AM

Cat. No. 5119

Calcein AM C46H46N2O23

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Chemical Name: N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester

Biological Activity

Cell permeable, non fluorescent compound; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells. Used for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.

Technical Data

M.Wt:
994.86
Formula:
C46H46N2O23
Solubility:
Soluble to 100 mM in DMSO
Purity:
>95 %
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

References are publications that support the products' biological activity.

Kuehn et al (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 286 391. PMID: 20980255.

Lazarowski et al (1997) Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 272 24328. PMID: 9305892.

Bakos et al (1996) Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 271 12322. PMID: 8647833.

De Gendt et al (1996) The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 249 189. PMID: 8737602.

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Citations are publications that use Tocris products.

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Keywords: Calcein AM, supplier, CAS, 148504-34-1, fluorescent, probes, agents, general, cells, viability, chemotaxis, adhesion, multidrug, resistance, MDR, Tocris Bioscience, Fluorescent Probe products

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