Hoechst 33342

Cat. No. 5117

Hoechst 33342 C27H28N6O.3HCl [23491-52-3]

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Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride

Biological Activity

Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. Used to quantify DNA in viable cells.

Technical Data

M.Wt:
561.93
Formula:
C27H28N6O.3HCl
Solubility:
Soluble to 100 mM in water and to 50 mM in DMSO
Purity:
>98 %
Storage:
Store at -20°C
CAS No:
23491-52-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

References are publications that support the products' biological activity.

Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158. PMID: 2449244.

Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136. PMID: 7028425.

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Keywords: Hoechst 33342, supplier, DNA, stains, fluorescent, dyes, nucleic, acid, cell, permeable, Tocris Bioscience, Fluorescent Dye products

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