Hoechst 33342

Cat. No. 5117

Hoechst 33342 C27H28N6O.3HCl [23491-52-3]

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Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride

Biological Activity

Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. Used to quantify DNA in viable cells.

Technical Data

Soluble to 100 mM in water and to 50 mM in DMSO
>98 %
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Safety Data Sheet

Certificate of Analysis: View current batch
Safety Data Sheet: View Safety Data Sheet

Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158. PMID: 2449244.

Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136. PMID: 7028425.

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Keywords: Hoechst 33342, supplier, DNA, stains, fluorescent, dyes, nucleic, acid, cell, permeable, Tocris Bioscience, Fluorescent Dyes, Labels & Stain products

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