Cat. No. 4366
Chemical Name: 3-Chloro-N-[trans-4-(methylamino)cy
Biological ActivityPotent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine action at the Smo receptor. Potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM). Induces pathway activation independently of Ptch proteins. Putative inhibitor of a cellular component required for Hedgehog signaling. Enhances neuronal differentiation of iPSCs into dopaminergic neurons.
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All Tocris products are intended for laboratory research use only.
Certificate of Analysis / Product Datasheet / Safety Data Sheet
Mak et al (2012) Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage. Stem Cells Int. 2012 140427. PMID: 22567022.
Stanton et al (2009) A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat.Chem.Biol. 5 154. PMID: 19151731.
Chen et al (2002) Small molecule modulation of Smoothened activity. Proc.Natl.Acad.Sci.USA. 99 14071. PMID: 12391318.
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Citations are publications that use Tocris products. Selected citations for SAG include:
Xia et al (2016) Transcriptional comparison of human induced and primary midbrain dopaminergic neurons. Sci.Rep. 6 20270. PMID: 26842779.
Cortes et al (2015) Accumulation of the Vitamin D Precursor Cholecalciferol Antagonizes Hedgehog Signaling to Impair Hemogenic Endothelium Formation. Nat Chem Biol 5 471. PMID: 26365513.
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Keywords: SAG, supplier, Potent, Smoothened, Smo, receptors, agonists, activates, Hedgehog, signaling, pathway, activator, enhancers, neuronal, differentiation, iPCs, inducible, pluripotent, stem, cells, dopaminergic, neurons, neurones, Tocris Bioscience, Smoothened Receptor Agonist products
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