Cat. No. 3439
Chemical Name: 1-Hexadecyl-2-methyl-3-(phenylmethy
Biological ActivityEukaryotic elongation factor 2 (eEF-2) kinase (CaMK III) inhibitor (IC50 = 60 nM) that displays 125-fold, > 1300-fold and > 1500-fold selectivity over PKC, PKA and CaMK II respectively. Decreases the viability of a variety of cancer cell lines (IC50 values are 0.7 - 4.8 μM) and blocks G1/S cell cycle progression. Shown to induce eEF-2 phosphorylation in cancer cells. Also an effective antibacterial agent; inhibits histidine protein kinase in vitro (IC50 = 6.6 μM) and in vivo.
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Certificate of Analysis / Product Datasheet / Safety Datasheet
References are publications that support the products' biological activity.
Chen (2011) 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) induces phosphorylation of eukaryotic elongation factor-2 (eEF2): a cautionary note on the anticancer mechanism of an eEF2 kinase inhibitor. J.Biol.Chem. 286 43951. PMID: 22020937.
Arora et al (2004) P-glycoprotein mediates resistance to histidine kinase inhibitors. Mol.Pharmacol. 66 460. PMID: 15322237.
Arora et al (2003) Identification and characterization of an inhibitor of eukaryotic elongation factor 2 kinase against human cancer cell lines. Cancer Res. 63 6894. PMID: 14583488.
Yamamoto et al (2001) Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis. Biosci.Biotechnol.Biochem. 65 2306. PMID: 11758928.
If you know of a relevant reference for NH 125 please let us know.
Citations are publications that use Tocris products. Selected citations for NH 125 include:
Kim et al (2015) Identification of an Antimicrobial Agent Effective against Methicillin-Resistant Staphylococcus aureus Persisters Using a Fluorescence-Based Screening Strategy. PLoS One 10 e0127640. PMID: 26039584.
Cheng et al (2013) Integrated regulation of autophagy and apoptosis by EEF2K controls cellular fate and modulates the efficacy of curcumin and velcade against tumor cells. Autophagy 9 208. PMID: 23182879.
Francis et al (2013) Mechanistic insight into inhibition of two-component system signaling. Medchemcomm 4 269. PMID: 23336064.
Do you know of a great paper that uses NH 125 from Tocris? If so please let us know.
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Keywords: NH 125, supplier, CaM, kinase, III, CAMK, eEF-2, inhibitors, inhibits, Histidine, protein, kinases, Calmodulin-Activated, calmodulin-dependent, Protein, NH125, Tocris Bioscience, Calmodulin-Dependent Kinase Inhibitor products
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