Biocytin

Cat. No. 3349

Biocytin C16H28N4O4S [576-19-2]

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Chemical Name: N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine

Biological Activity

Versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

Technical Data

M.Wt:
372.48
Formula:
C16H28N4O4S
Solubility:
Soluble to 50 mM in water with gentle warming
Storage:
Store at -20°C
CAS No:
576-19-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

Certificate of Analysis / Product Datasheet
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MacNeil and Gaul (2008) Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 506 6. PMID: 17990268.

Roth et al (2004) Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 478 35. PMID: 15334648.

Kobbert et al (2000) Current concepts in neuroanatomical tracing. Prog.Neurobiol. 62 327. PMID: 10856608.

If you know of a relevant reference for this product please let us know.

Citations are publications that use Tocris products. Selected citations for Biocytin include:

Robinson and Wyatt (2015) Identification and structure solution of fragment hits against kinetoplastid N-myristoyltransferase. Nature 71 586. PMID: 25945713.

Cunningham et al (2014) hPSC-derived maturing GABAergic interneurons ameliorate seizures and abnormal behavior in epileptic mice. Cell Stem Cell 15 559. PMID: 25517465.

Dettmann et al (2014) Isolation, expansion and transplantation of postnatal murine progenitor cells of the enteric nervous system. PLoS One 9 e97792. PMID: 24871092.

Xia et al (2014) Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons. PLoS One 9 e100968. PMID: 24971996.

Tsai et al (2012) Autistic-like behaviour and cerebellar dysfunction in Purkinje cell Tsc1 mutant mice. MBio 488 647. PMID: 22763451.

Jerome et al (2011) Parallel optical control of spatiotemporal neuronal spike activity using high-speed digital light processing. Front Syst Neurosci 5 70. PMID: 21904526.

Lam et al (2011) Leptin does not directly affect CNS serotonin neurons to influence appetite. Acta Crystallogr F Struct Biol Commun 13 584. PMID: 21531340.

Do you know of a great paper that uses Biocytin from Tocris? If so please let us know.show all ▼show less ▲

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Keywords: Biocytin, supplier, Versatile, marker, neuroanatomical, investigations, reagents, anterograde, retrograde, Tocris Bioscience, Biochemicals and Molecular Biology products

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