Cat. No. 3257

5-Fluorouracil C4H3FN2O2 [51-21-8]

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Alternative Name: 5-FU

Chemical Name: 5-Fluoro-2,4-(1H,3H)-pyrimidinedione

Biological Activity

Anticancer agent. Metabolized to form fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine (FUTP). FdUMP inhibits thymidylate synthase, causing a reduction in dTMP synthesis. FUTP and FdUTP are misincorporated into RNA and DNA respectively.

Technical Data

Soluble to 100 mM in DMSO and to 10 mM in ethanol
>99 %
Store at +4°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

References are publications that support the products' biological activity.

Longley et al (2003) 5-Fluorouracil: mechanisms of action and clinical strategies. Nat.Rev.Cancer 3 330. PMID: 12724731.

Peters et al (2002) Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Biochim.Biophys.Acta. 1587 194. PMID: 12084461.

Ghoshal and Jacob (1997) An alternative molecular mechanism of action of 5-fluorouracil, a potent anticancer drug. Biochem.Pharmacol. 53 1569. PMID: 9264308.

If you know of a relevant reference for 5-Fluorouracil please let us know.

Citations are publications that use Tocris products. Selected citations for 5-Fluorouracil include:

Fukushima et al (2015) Atonal homolog 1 protein stabilized by tumor necrosis factor α induces high malignant potential in colon cancer cell line. Cancer Sci 106 1000. PMID: 26017781.

Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641. PMID: 24265703.

Do you know of a great paper that uses 5-Fluorouracil from Tocris? If so please let us know.

View Related Products by Product Action

Keywords: 5-Fluorouracil, supplier, inhibitors, inhibits, RNA, DNA, synthesis, thymidylate, synthetases, Synthases, antimetabolites, 5-FU, chemotherapeutics, Tocris Bioscience, DNA, RNA and Protein Synthesis Inhibitor products

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