BIO

Cat. No. 3194

BIO C16H10BrN3O2 [667463-62-9]

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Alternative Name: 6BIO

Chemical Name: (2'Z,3'E)-6-Bromoindirubin-3'-oxime

Biological Activity

Potent and selective, ATP-competitive glycogen synthase kinase-3 (GSK-3) inhibitor (IC50 values are 5, 83, 300, 320 and > 10 000 nM at GSK-3, CDK5/p25, CDK2/cyclin A, CDK1/cyclin B and other common kinases respectively). Maintains self-renewal and pluripotency in embryonic stem cells via activation of the Wnt signaling pathway in vitro; also promotes proliferation and dedifferentiation in cardiomyocytes. Negative control available (Cat. No. 3873).

Technical Data

M.Wt:
356.17
Formula:
C16H10BrN3O2
Solubility:
Soluble to 10 mM in DMSO and to 10 mM in ethanol
Purity:
>98 %
Storage:
Store at +4°C
CAS No:
667463-62-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

References are publications that support the products' biological activity.

Tseng et al (2006) The GSK-3 inhibitor BIO promotes proliferation in mammalian cardiomyocytes. Chem.Biol. 13 957. PMID: 16984885.

Polychronopoulos et al (2004) Structural basis for the synthesis of indirubins as potent and selective inhibtors of glycogen synthase kinase-3 and cyclin dependent kinases. J.Med.Chem. 47 935. PMID: 14761195.

Meijer et al (2003) GSK-3-selective inhibitors derived from tyrian purple indirubins. Chem.Biol. 10 1255. PMID: 14700633.

If you know of a relevant reference for BIO please let us know.

Citations are publications that use Tocris products. Selected citations for BIO include:

Efthymiou et al (2015) Rescue of an in vitro neuron phenotype identified in Niemann-Pick disease, type C1 induced pluripotent stem cell-derived neurons by modulating the WNT pathway and calcium signaling. Elife 4 230. PMID: 25637190.

Lindström et al (2015) Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Nat Chem Biol 3 e04000. PMID: 25647637.

Penas et al (2015) GSK3 inhibitors stabilize Wee1 and reduce cerebellar granule cell progenitor proliferation. Nat Commun 14 417. PMID: 25616418.

Pronobis et al (2015) A novel GSK3-regulated APC:Axin interaction regulates Wnt signaling by driving a catalytic cycle of efficient βcatenin destruction. Cell 4 e08022. PMID: 26393419.

Rhim et al (2015) A High-content screen identifies compounds promoting the neuronal differentiation and the midbrain dopamine neuron specification of human neural progenitor cells. Elife 5 16237. PMID: 26542303.

Thorne et al (2015) GSK-3 modulates cellular responses to a broad spectrum of kinase inhibitors. Stem Cells Transl Med 11 58. PMID: 25402767.

Zhang et al (2015) GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS Biol 13 e1002129. PMID: 25860027.

Berger et al (2014) PAK-dependent STAT5 serine phosphorylation is required for BCR-ABL-induced leukemogenesis. Cell Cycle 28 629. PMID: 24263804.

Lu et al (2014) Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway. Sci Rep 5 5368. PMID: 25371059.

Guo et al (2012) Slug and Sox9 cooperatively determine the mammary stem cell state. World J Gastroenterol 148 1015. PMID: 22385965.

Do you know of a great paper that uses BIO from Tocris? If so please let us know.show all ▼show less ▲

View Related Products by Product Action

Keywords: BIO, supplier, Potent, selective, GSK-3, inhibitors, inhibits, Glycogen, Synthase, Kinases, 3, Carbohydrate, Metabolism, stem, cells, Tocris Bioscience, Glycogen Synthase Kinase 3 Inhibitor products

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