Cat. No. 1228
Chemical Name: [5-(2-Thienylcarbonyl)-1H-benzimida
Biological ActivityMicrotubule inhibitor; inhibits mitosis. Also inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Certificate of Analysis / Product Datasheet / Safety Data Sheet
Lin et al (2014) Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 3 e04766. PMID: 25497837.
Mizushima et al (2010) Methods in mammalian autophagy research. Cell 140 313. PMID: 20144757.
Webb et al (2004) Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 36 2541. PMID: 15325591.
Vasquez et al (1997) Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 8 973. PMID: 9201709.
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Citations are publications that use Tocris products. Selected citations for Nocodazole include:
Guo et al (2016) Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis. Front Cell Neurosci 18 202. PMID: 26655835.
Lin et al (2015) Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion. Nat Commun 6 18577. PMID: 26124177.
Tauchman et al (2015) Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Cell Biol 6 10036. PMID: 26620470.
Hutchins and Wray (2014) Capture of microtubule plus-ends at the actin cortex promotes axophilic neuronal migration by enhancing microtubule tension in the leading process. Methods Mol Biol 8 400. PMID: 25505874.
Seese et al (2012) LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice. J Neurosci 32 7403. PMID: 22623686.
Rood et al (2011) Methods for purification of proteins associated with cellular poly(ADP-ribose) and PARP-specific poly(ADP-ribose). Anal Biochem 780 153. PMID: 21870260.
Schapitz et al (2010) Neuroligin 1 is dynamically exchanged at postsynaptic sites. Oncotarget 30 12733. PMID: 20861378.
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Keywords: Nocodazole, supplier, Microtubule, inhibitors, inhibits, Tau, Tubulin, Microtubules, Mitosis, crispr, cas9, homology-directed, repair, HDR, Tocris Bioscience, Microtubule products
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December 13 - 15, 2016