SR 95531 hydrobromide

Cat. No. 1262

SR 95531 hydrobromide C15H17N3O3.HBr [104104-50-9]

Price and Availability

For SR 95531 hydrobromide pricing & availability please select your country from the drop down menu:
Submit
By clicking submit you agree to accept a cookie from Tocris Bioscience. For details, please read our privacy and cookie policy.

Alternative Name: Gabazine

Chemical Name: 6-Imino-3-(4-methoxyphenyl)-1(6H)-pyridazinebutanoic acid hydrobromide

Biological Activity

Selective, competitive GABAA receptor antagonist. Displaces [3H]-GABA from rat brain membranes with a Ki of 150 nM. Unlike bicuculline, selectively antagonizes GABA-induced Cl- currents with little action on pentobarbitone-induced currents. Also acts as a low affinity glycine receptor antagonist.

Technical Data

M.Wt:
368.23
Formula:
C15H17N3O3.HBr
Solubility:
Soluble to 25 mM in water and to 100 mM in DMSO
Purity:
>98 %
Storage:
Store at RT
CAS No:
104104-50-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Data Sheet

Certificate of Analysis / Product Datasheet
Select another batch:

Beato et al (2007) The kinetics of inhibition of rat recombinant heterotrimeric α1β glycine receptors by the low affinity antagonist SR-95531. J.Physiol. 580 171. PMID: 17218350.

Uchida et al (1996) The differential antagonism by bicuculline and SR95531 of pentobarbitone-induced currents in cultured hippocampal neurons. Eur.J.Pharmacol. 307 89. PMID: 8831109.

Heaulme et al (1986) Biochemical characterization of the interaction of three pyridazinyl-GABA derivatives with the GABAA receptor site. Brain Res. 384 224. PMID: 3022866.

If you know of a relevant reference for this product please let us know.

Citations are publications that use Tocris products. Selected citations for SR 95531 hydrobromide include:

Miguel Angel Garcia-Bereguiain et al (2016) Spontaneous release regulates synaptic scaling in the embryonic spinal network in vivo. The Journal of Neuroscience 6 7268. PMID: 27383600.

Morton et al (2016) Electrophysiological Assessment of Serotonin and GABA Neuron Function in the Dorsal Raphe during the Third Trimester Equivalent Developmental Period in Mice(1,2,3). Proc Natl Acad Sci U S A 2. PMID: 26730407.

Philippart et al (2016) Differential somatic Ca2+ channel profile in midbrain dopaminergic neurons. The Journal of Neuroscience 6 7234. PMID: 27383597.

Piet et al (2016) Vasoactive Intestinal Peptide Excites GnRH Neurons in Male and Female Mice. Endocrinology. 157 3621. PMID: 27501185 .

Abdi et al (2015) Prototypic and arkypallidal neurons in the dopamine-intact external globus pallidus. J Neurosci 35 6667. PMID: 25926446.

Astorga et al (2015) An excitatory GABA loop operating in vivo. Front Cell Neurosci 9 275. PMID: 26236197.

Baculis and Valenzuela (2015) Ethanol exposure during the third trimester equivalent does not affect GABAA or AMPA receptor-mediated spontaneous synaptic transmission in rat CA3 pyramidal neurons. J Neurophysiol 14 19. PMID: 26627643.

Baltz and Voigt (2015) Interaction of electrically evoked activity with intrinsic dynamics of cultured cortical networks with and without functional fast GABAergic synaptic transmission. Front Cell Neurosci 9 272. PMID: 26236196.

Bell et al (2015) Acetylcholine release in mouse hippocampal CA1 preferentially activates inhibitory-selective interneurons via α4β2* nicotinic receptor activation. J Neurosci 9 115. PMID: 25918499.

Bocchio et al (2015) Increased serotonin transporter expression reduces fear and recruitment of parvalbumin interneurons of the amygdala. Neuropsychopharmacology 40 3015. PMID: 26052039.

Choe et al (2015) High salt intake increases blood pressure via BDNF-mediated downregulation of KCC2 and impaired baroreflex inhibition of vasopressin neurons. Front Cell Neurosci 85 549. PMID: 25619659.

Collot et al (2015) CaRuby-Nano: a novel high affinity calcium probe for dual color imaging. Front Cell Neurosci 4. PMID: 25824291.

Cramer et al (2015) Abnormal excitability and episodic low-frequency oscillations in the cerebral cortex of the tottering mouse. Physiol Rep 35 5664. PMID: 25855180.

Duménieu et al (2015) Afterhyperpolarization (AHP) regulates the frequency and timing of action potentials in the mitral cells of the olfactory bulb: role of olfactory experience. PLoS One 3. PMID: 26019289.

Elgueta et al (2015) Acetylcholine induces GABA release onto rod bipolar cells through heteromeric nicotinic receptors expressed in A17 amacrine cells. Microvasc Res 9 6. PMID: 25709566.

Fan et al (2015) Stretch induced hyperexcitability of mice callosal pathway. J Neurophysiol 9 292. PMID: 26300729.

Hirono et al (2015) BK Channels Localize to the Paranodal Junction and Regulate Action Potentials in Myelinated Axons of Cerebellar Purkinje Cells. Eneuro 35 7082. PMID: 25948259.

Kardamakis et al (2015) Tectal microcircuit generating visual selection commands on gaze-controlling neurons. PLoS One 112 E1956. PMID: 25825743.

Kim et al (2015) An excitatory amacrine cell detects object motion and provides feature-selective input to ganglion cells in the mouse retina. Elife 4. PMID: 25988808.

Laprell et al (2015) Optical control of NMDA receptors with a diffusible photoswitch. Front Cell Neurosci 6 8076. PMID: 26311290.

Liu et al (2015) Muscarinic receptors modulate dendrodendritic inhibitory synapses to sculpt glomerular output. J Negat Results Biomed 35 5680. PMID: 25855181.

Mapelli et al (2015) The effect of desflurane on neuronal communication at a central synapse. Nat Commun 10 e0123534. PMID: 25849222.

Murphy-Baum and Taylor (2015) The Synaptic and Morphological Basis of Orientation Selectivity in a Polyaxonal Amacrine Cell of the Rabbit Retina. Brain 35 13336. PMID: 26424882.

Polito et al (2015) Selective Effects of PDE10A Inhibitors on Striatopallidal Neurons Require Phosphatase Inhibition by DARPP-32(1,2,3). J Neurosci 2. PMID: 26465004.

Saunders et al (2015) Corelease of acetylcholine and GABA from cholinergic forebrain neurons. Elife 4. PMID: 25723967.

Scharinger et al (2015) Cell-type-specific tuning of Cav1.3 Ca(2+)-channels by a C-terminal automodulatory domain. Elife 9 309. PMID: 26379493.

Tabatadze et al (2015) Sex Differences in Molecular Signaling at Inhibitory Synapses in the Hippocampus. J Neurosci 35 11252. PMID: 26269634.

Wang et al (2015) RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep 5 17383. PMID: 26619789.

Wang et al (2015) Apamin Boosting of Synaptic Potentials in CaV2.3 R-Type Ca2+ Channel Null Mice. Eneuro 10 e0139332. PMID: 26418566.

Winkelmann et al (2015) Chloride transporter KCC2-dependent neuroprotection depends on the N-terminal protein domain. Cell Death Dis 6 e1776. PMID: 26043076.

Albrecht et al (2014) Inhibitory projections from the ventral nucleus of the trapezoid body to the medial nucleus of the trapezoid body in the mouse. Front Neural Circuits 8 83. PMID: 25120436.

Chuhma et al (2014) Dopamine neurons control striatal cholinergic neurons via regionally heterogeneous dopamine and glutamate signaling. PLoS One 81 901. PMID: 24559678.

Diaz et al (2014) Moderate Alcohol Exposure during the Rat Equivalent to the Third Trimester of Human Pregnancy Alters Regulation of GABAA Receptor-Mediated Synaptic Transmission by Dopamine in the Basolateral Amygdala. J Neurosci 2 46. PMID: 24904907.

Dragicevic et al (2014) Cav1.3 channels control D2-autoreceptor responses via NCS-1 in substantia nigra dopamine neurons. Neuron 137 2287. PMID: 24934288.

Goddard et al (2014) Spatially reciprocal inhibition of inhibition within a stimulus selection network in the avian midbrain. PLoS One 9 e85865. PMID: 24465755.

Grimes et al (2014) Cross-synaptic synchrony and transmission of signal and noise across the mouse retina. Elife 3 e03892. PMID: 25180102.

Ivanov et al (2014) Glycolysis and oxidative phosphorylation in neurons and astrocytes during network activity in hippocampal slices. J Cereb Blood Flow Metab 34 397. PMID: 24326389.

Jones et al (2014) Neonatal NMDA receptor blockade disrupts spike timing and glutamatergic synapses in fast spiking interneurons in a NMDA receptor hypofunction model of schizophrenia. Nature 9 e109303. PMID: 25290690.

Kubota et al (2014) mGluR1-mediated excitation of cerebellar GABAergic interneurons requires both G protein-dependent and Src-ERK1/2-dependent signaling pathways. PLoS One 9 e106316. PMID: 25181481.

Lee et al (2014) Synapse elimination and learning rules co-regulated by MHC class I H2-Db. Nat Neurosci 509 195. PMID: 24695230.

Mariqueo et al (2014) Effects of ethanol on glycinergic synaptic currents in mouse spinal cord neurons. Front Pediatr 111 1940. PMID: 24572089.

Mills et al (2014) Three distinct blue-green color pathways in a mammalian retina. J Neurosci 34 1760. PMID: 24478358.

Muralidhar et al (2014) Synaptic and cellular organization of layer 1 of the developing rat somatosensory cortex. Front Neuroanat 7 52. PMID: 24474905.

Nieus et al (2014) Regulation of output spike patterns by phasic inhibition in cerebellar granule cells. Front Cell Neurosci 8 246. PMID: 25202237.

Ostrowski et al (2014) Depressed GABA and glutamate synaptic signaling by 5-HT1A receptors in the nucleus tractus solitarii and their role in cardiorespiratory function. J Neurosci 111 2493. PMID: 24671532.

Schönberger et al (2014) Lamina-specific contribution of glutamatergic and GABAergic potentials to hippocampal sharp wave-ripple complexes. J Cereb Blood Flow Metab 8 103. PMID: 25202239.

Simon et al (2014) Gap junction networks can generate both ripple-like and fast ripple-like oscillations. Eur J Neurosci 39 46. PMID: 24118191.

Straub et al (2014) CRISPR/Cas9-mediated gene knock-down in post-mitotic neurons. J Neurophysiol 9 e105584. PMID: 25140704.

Varodayan and Harrison (2014) HSF1 transcriptional activity mediates alcohol induction of Vamp2 expression and GABA release. J Neurophysiol 7 89. PMID: 24376402.

Akrouh and Kerschensteiner (2013) Intersecting circuits generate precisely patterned retinal waves. Neuron 79 322. PMID: 23830830.

Berlinguer-Palmini et al (2013) GPR35 activation reduces Ca2+ transients and contributes to the kynurenic acid-dependent reduction of synaptic activity at CA3-CA1 synapses. PLoS One 8 e82180. PMID: 24312407.

Gao and Derbenev (2013) Synaptic and extrasynaptic transmission of kidney-related neurons in the rostral ventrolateral medulla. J Neurosci 110 2637. PMID: 24027107.

Gkogkas et al (2013) Autism-related deficits via dysregulated eIF4E-dependent translational control. Proc Natl Acad Sci U S A 493 371. PMID: 23172145.

Hoshi et al (2013) Properties of the ON bistratified ganglion cell in the rabbit retina. J Comp Neurol 521 1497. PMID: 23047654.

Maejima et al (2013) Postnatal loss of P/Q-type channels confined to rhombic-lip-derived neurons alters synaptic transmission at the parallel fiber to purkinje cell synapse and replicates genomic Cacna1a mutation phenotype of ataxia and seizures in mice. J Neurosci 33 5162. PMID: 23516282.

Nguyen et al (2013) GABA(A) receptors implicated in REM sleep control express a benzodiazepine binding site. J Biol Chem 1527 131. PMID: 23835499.

Peng et al (2013) A reorganized GABAergic circuit in a model of epilepsy: evidence from optogenetic labeling and stimulation of somatostatin interneurons. PLoS One 33 14392. PMID: 24005292.

Szemes et al (2013) Weaker control of the electrical properties of cerebellar granule cells by tonically active GABAA receptors in the Ts65Dn mouse model of Down's syndrome. Mol Brain 6 33. PMID: 23870245.

Ufartes et al (2013) Behavioural and functional characterization of Kv10.1 (Eag1) knockout mice. Hum Mol Genet 22 2247. PMID: 23424202.

Akerboom et al (2012) Optimization of a GCaMP calcium indicator for neural activity imaging. J Neurosci 32 13819. PMID: 23035093.

Austgen et al (2012) 5-hydroxytryptamine 2C receptors tonically augment synaptic currents in the nucleus tractus solitarii. Cereb Cortex 108 2292. PMID: 22855775.

Chacón et al (2012) Focal adhesion kinase regulates actin nucleation and neuronal filopodia formation during axonal growth. Brain Res 139 3200. PMID: 22872087.

Fortin et al (2012) Brain-derived neurotrophic factor activation of CaM-kinase kinase via transient receptor potential canonical channels induces the translation and synaptic incorporation of GluA1-containing calcium-permeable AMPA receptors. J Neurosci 32 8127. PMID: 22699894.

Guli et al (2012) ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses. Neural Plast 2012 237913. PMID: 22792490.

Hirono et al (2012) Cerebellar globular cells receive monoaminergic excitation and monosynaptic inhibition from Purkinje cells. PLoS One 7 e29663. PMID: 22235322.

Kolind et al (2012) Opposing Effects of Intrinsic Conductance and Correlated Synaptic Input on V-Fluctuations during Network Activity. Front Comput Neurosci 6 40. PMID: 22783184.

Lee and Sherman (2012) Intrinsic modulators of auditory thalamocortical transmission. Hear Res 287 43. PMID: 22726616.

Lethbridge et al (2012) Olfactory bulb glomerular NMDA receptors mediate olfactory nerve potentiation and odor preference learning in the neonate rat. Mol Pain 7 e35024. PMID: 22496886.

Lonchamp et al (2012) Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex. PLoS One 7 e30180. PMID: 22276158.

Takesian et al (2012) Age-dependent effect of hearing loss on cortical inhibitory synapse function. J Neurophysiol 107 937. PMID: 22090457.

Allen et al (2011) The SK2-long isoform directs synaptic localization and function of SK2-containing channels. Nat Neurosci 14 744. PMID: 21602822.

Allen et al (2011) SK2 channels are neuroprotective for ischemia-induced neuronal cell death. J Neurosci 31 2302. PMID: 21712833.

Austgen et al (2011) Hydrogen sulfide augments synaptic neurotransmission in the nucleus of the solitary tract. J Neurophysiol 106 1822. PMID: 21734104.

Biase et al (2011) NMDA receptor signaling in oligodendrocyte progenitors is not required for oligodendrogenesis and myelination. J Neurophysiol 31 12650. PMID: 21880926.

Chu et al (2011) Synaptic responses evoked by tactile stimuli in Purkinje cells in mouse cerebellar cortex Crus II in vivo. PLoS One 6 e22752. PMID: 21818384.

George et al (2011) A diversity of synaptic filters are created by temporal summation of excitation and inhibition. J Neurosci 31 14721. PMID: 21994388.

Goldberg et al (2011) Rapid developmental maturation of neocortical FS cell intrinsic excitability. Front Cell Neurosci 21 666. PMID: 20705896.

Randall et al (2011) Fast oscillatory activity induced by kainate receptor activation in the rat basolateral amygdala in vitro. Eur J Neurosci 33 914. PMID: 21255131.

Reali et al (2011) GABAergic signalling in a neurogenic niche of the turtle spinal cord. J Physiol 589 5633. PMID: 21911613.

Wei et al (2011) Development of asymmetric inhibition underlying direction selectivity in the retina. J Neurophysiol 469 402. PMID: 21131947.

Zschenderlein et al (2011) Capsaicin-induced changes in LTP in the lateral amygdala are mediated by TRPV1. PLoS One 6 e16116. PMID: 21249195.

Biase et al (2010) Excitability and synaptic communication within the oligodendrocyte lineage. J Neurosci 30 3600. PMID: 20219994.

Kim and Alger (2010) Reduction in endocannabinoid tone is a homeostatic mechanism for specific inhibitory synapses. Nat Neurosci 13 592. PMID: 20348918.

Mapelli et al (2010) High-Pass Filtering and Dynamic Gain Regulation Enhance Vertical Bursts Transmission along the Mossy Fiber Pathway of Cerebellum. Nature 4 14. PMID: 20577586.

Nikolenko et al (2010) A portable laser photostimulation and imaging microscope. J Neural Eng 7 45001. PMID: 20644244.

Noh et al (2010) Glutamate co-release at GABA/glycinergic synapses is crucial for the refinement of an inhibitory map. PLoS One 13 232. PMID: 20081852.

Oh et al (2010) Substitution of 5-HT1A receptor signaling by a light-activated G protein-coupled receptor. Front Neural Circuits 285 30825. PMID: 20643652.

Person and Raman (2010) Deactivation of L-type Ca current by inhibition controls LTP at excitatory synapses in the cerebellar nuclei. Neuron 66 550. PMID: 20510859.

Tani et al (2010) Glutamine is required for persistent epileptiform activity in the disinhibited neocortical brain slice. J Neurosci 30 1288. PMID: 20107056.

Torborg et al (2010) Control of CA3 output by feedforward inhibition despite developmental changes in the excitation-inhibition balance. J Neurosci 30 15628. PMID: 21084618.

Venkataramani and Taylor (2010) Orientation selectivity in rabbit retinal ganglion cells is mediated by presynaptic inhibition. J Neurosci 30 15664. PMID: 21084622.

Babu et al (2009) Synaptic network activity induces neuronal differentiation of adult hippocampal precursor cells through BDNF signaling. Front Neurosci 3 49. PMID: 20582276.

Brozoski et al (2009) Differential endocannabinoid regulation of baroreflex-evoked sympathoinhibition in normotensive versus hypertensive rats. Auton Neurosci 150 82. PMID: 19464961.

Chuhma et al (2009) Dopamine neuron glutamate cotransmission: frequency-dependent modulation in the mesoventromedial projection. Neuroscience 164 1068. PMID: 19729052.

Li et al (2009) Activity-dependent modulation of glutamatergic signaling in the developing rat dorsal horn by early tissue injury. Cereb Cortex 102 2208. PMID: 19675290.

Shiratsuchi et al (2009) Propofol inhibits pressure-stimulated macrophage phagocytosis via the GABAA receptor and dysregulation of p130cas phosphorylation. Am J Physiol Cell Physiol 296 C1400. PMID: 19357231.

Wood et al (2009) Synaptic circuit abnormalities of motor-frontal layer 2/3 pyramidal neurons in an RNA interference model of methyl-CpG-binding protein 2 deficiency. Chem Senses 29 12440. PMID: 19812320.

Darbin and Wichmann (2008) Effects of striatal GABA A-receptor blockade on striatal and cortical activity in monkeys. Front Cell Neurosci 99 1294. PMID: 18216224.

Fanselow et al (2008) Selective, state-dependent activation of somatostatin-expressing inhibitory interneurons in mouse neocortex. J Neurophysiol 100 2640. PMID: 18799598.

Kim and Tsien (2008) Synapse-specific adaptations to inactivity in hippocampal circuits achieve homeostatic gain control while dampening network reverberation. Neuron 58 925. PMID: 18579082.

Kotak et al (2008) Hearing loss prevents the maturation of GABAergic transmission in the auditory cortex. Cereb Cortex 18 2098. PMID: 18222937.

Maher and Westbrook (2008) Co-transmission of dopamine and GABA in periglomerular cells. J Neurophysiol 99 1559. PMID: 18216231.

Shin et al (2008) Dendritic glutamate release produces autocrine activation of mGluR1 in cerebellar Purkinje cells. J Neurosci 105 746. PMID: 18174329.

Fortin and Levine (2007) Differential effects of endocannabinoids on glutamatergic and GABAergic inputs to layer 5 pyramidal neurons. Development 17 163. PMID: 16467564.

Young and Sun (2007) Long-term modifications in the strength of excitatory associative inputs in the piriform cortex. Neuron 32 783. PMID: 17634388.

Ziskin et al (2007) Vesicular release of glutamate from unmyelinated axons in white matter. Front Integr Neurosci 10 321. PMID: 17293857.

Ince-Dunn et al (2006) Regulation of thalamocortical patterning and synaptic maturation by NeuroD2. Neuron 49 683. PMID: 16504944.

Singh et al (2006) Altered balance of glutamatergic/GABAergic synaptic input and associated changes in dendrite morphology after BDNF expression in BDNF-deficient hippocampal neurons. Nature 26 7189. PMID: 16822976.

Foeller et al (2005) Inhibitory sharpening of receptive fields contributes to whisker map plasticity in rat somatosensory cortex. J Neurophysiol 94 4387. PMID: 16162832.

Ying and Goldstein (2005) Propofol suppresses synaptic responsiveness of somatosensory relay neurons to excitatory input by potentiating GABA(A) receptor chloride channels. J Neurosci 1 2. PMID: 15813991.

Huang et al (2004) Astrocyte glutamate transporters regulate metabotropic glutamate receptor-mediated excitation of hippocampal interneurons. J Neurosci 24 4551. PMID: 15140926.

Labrakakis et al (2003) Localization and function of ATP and GABAA receptors expressed by nociceptors and other postnatal sensory neurons in rat. J Physiol 549 131. PMID: 12665615.

Do you know of a great paper that uses SR 95531 hydrobromide from Tocris? If so please let us know.show all ▼show less ▲

View Related Products by Target

View Related Products by Product Action

Keywords: SR 95531 hydrobromide, supplier, Selective, competitive, GABAA, receptor, antagonists, Receptors, SR95531, hydrobromide, Tocris Bioscience, GABAA Receptor Antagonist products

Quick Order

Find multiple products by catalog number

divider line

Pain Research Product Guide

Pain Research Product Guide

Our Pain guide highlights over 280 products for Pain research. Request copy or view PDF today.

divider line

GABA Review

Written by I. Martin et al

GABA Receptors Scientific Review

Our GABA review gives an overview of the structure and function of the GABA receptors, as well as highlighting pharmacological ligands for their study. Request copy or view PDF today.

divider line

Peripheral Sensitization

Written by Grant D. Nicol and Michael R. Vasko

Pain Life Science Poster

Our Peripheral Sensitization poster gives a summary of the excitatory and inhibitory signaling pathways involved in modulation of peripheral sensitization. Request copy or view PDF today.

divider line

Therapeutic Strategies in Schizophrenia

Written by J. Lieberman

Schizophrenia Life Science Poster

Our Schizophrenia poster higlights the targets and pathways representing the most promising strategies to pursue for novel drug development. Request copy today.

divider line

Mechanisms of Addiction

Addiction Life Science Poster

Written by Prof David Nutt, this poster summarizes the brain circuits and neurotransmitter systems that are affected by the main classes of addictive drugs. Request copy or view PDF today.

divider line

Bio-Techne Events

One Day Symposium

Bio-Techne Symposium 2017

Epigenetic Regulation of Stem Cell Differentiation

March 1, 2017

Amsterdam, The Netherlands